Navegando por Palavras-chave "Ligação Proteica"

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    Estudo Das Biointerações De Nanopartículas De Ouro Ultrapequenas: Influência Do Tamanho E Revestimento De Superfície Dissertação
    (Universidade Federal de São Paulo (UNIFESP), 2017-03-31) Knittel, Luiza Leite [UNIFESP]; Sousa, Alioscka Augusto Caldeira Araujo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Thus, it is possible to control the size, shape and surface chemistry of nanomaterials, aiming stable and inert structures, or even aiming to regulate the interactions of nanomaterials with proteins and cells. For example, studies showed that ultrasmall gold nanoparticles (NPs) «3 nm) coated with glutathione are excreted by the kidneys at the same time that it has a good retention in the tumor environment. Thus, this work presents an in vitro study of ultrasmall gold NPs behavior in biological fluid and the conditioning of this behavior to the physicochemical characteristics of the particles. In a first step, we studied the relation between the size of ultrasmall NPs coated with glutathione and its colloidal stability. ;~ In a second step, we seek to improve the surface chemistry of the preceding NPs, to produce new nanostructures with improved colloidal stability and greater resistance capacity to interaction with serum proteins. Therefore, we use zwitterionic surface molecules. In the third and final stage of this work, we modulated the surface chemistry with new synthetic tripeptide aiming the formulation of ultrasmall NPs that had a purposeful interaction with serum proteins. In order to establish a relation between the surface chemistry of NPs and the identity of proteins adsorbed therein, we will seek to identify via mass spectrometry, in a next step, the serum proteins that have a preferential interaction by the different NPs. In general, this work aims a better understanding of the relation between the physicochemical properties of ultrasmall gold NPs and their biointeraction in vitro, in order to predict and possibly control its bioresponses in vivo.