Navegando por Palavras-chave "Leydig cell"
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- ItemSomente MetadadadosCarbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring(Elsevier B.V., 2014-04-01) Andretta, Rhayza Roberta [UNIFESP]; Okada, Fatima Kazue [UNIFESP]; Paccola, Camila Cicconi [UNIFESP]; Stumpp, Taiza [UNIFESP]; Oliva, Samara Urban de [UNIFESP]; Miraglia, Sandra M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Carbamazepine (CBZ) is an anti-epileptic drug that acts on Leydig cells, affecting steroidogenesis and causes fetal malformation. the aim of this study was to investigate the effects of CBZ on male sexual maturation and other male parameters. Rat dams were treated with CBZ during pregnancy and breastfeeding. the anogenital distance (AGD) and the anogenital index (AGI) were obtained. Testicular descent and preputial separation were also evaluated. the offspring was euthanized at PND 41 and 63. the accessory glands were weighed and the testes were collected for histopathological, morphometric and sterological analyses. the numerical density of Leydig cells and hormone dosage were obtained. CBZ caused an increase of AGI and a delay of testicular descent and of preputial separation. CBZ also caused a decrease of testosterone level and of sperm count and an increase of abnormal sperm. These results indicate that CBZ delays puberty onset and affects steroidogenesis and sperm quality. (C) 2013 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosEffects of prenatal and lactation nicotine exposure on rat testicular interstitial tissue(Wiley-Blackwell, 2014-03-01) Paccola, Camila Cicconi [UNIFESP]; Neves, F. M. O. [UNIFESP]; Cipriano, I. [UNIFESP]; Stumpp, Taiza [UNIFESP]; Miraglia, Sandra Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Nicotine is largely consumed as a component of cigarettes. It induces apoptosis, interferes with endocrine function by changing the sex hormones secretion and leads to male infertility. Testosterone is produced from cholesterol by Leydig cells (LC), with the participation of testicular macrophages (MO). Thus, to investigate whether nicotine administration to pregnant and lactating rats changes cholesterol and sexual hormone levels and LC and MO populations of offspring, female rats received nicotine (2mg/kg/day) through osmotic minipumps from the first day of pregnancy up to the end of weaning. At 1, 30, 60 and 90days post-partum (dpp) the plasma cholesterol and testosterone levels were obtained, as well as the biometric, histopathological and stereological testicular parameters. Nicotine reduced the body weight, cholesterol levels and lipid droplet number in foetal LC at 1dpp. the number of apoptotic LC did not change in the offspring of nicotine group at any age studied. No alterations in the numerical densities of MO and LC occurred at 60 and 90dpp. Hypertrophy of mature LC and increase in cholesterol and testosterone levels were noted at 90dpp. in conclusion, nicotine when administered to rats throughout pregnancy and lactation induces morphofunctional alterations of foetal and mature LC and affects cholesterol and testosterone levels.
- ItemSomente MetadadadosEvaluation of the tubular and interstitial functions of the testis in 46,XY patients with ambiguous genitalia(Freund Publishing House Ltd, 2000-06-01) Stuchi-Perez, E. Gabas; Lukas-Croisier, C.; De Castro, M.; Baptista, MT Matias; Scolfaro, M. Ribeiro; Marques-De-Faria, A. P.; Hackel, C.; Maciel-Guerra, A. T.; Guerra, G.; Universidade Estadual de Campinas (UNICAMP); Ecole Normale Super; Universidade Federal de São Paulo (UNIFESP)Investigation of the origin of sexual ambiguity is complex. Although testicular function has traditionally been assessed only by examining the steroidogenic capacity of Leydig cells and spermatogenesis, it has recently been shown that the measurement of serum anti-Mullerian hormone (AMH) as a marker of Sertoli cell function may also help clinicians. The aim of this study was to evaluate both Leydig and Sertoli cell functions in 46,XY patients with intersex states in order to establish biochemical patterns that would help to reach an etiologic diagnosis. We measured serum androgens, AMH and gonadotropins in 24 patients with sexual ambiguity and XY karyotype: 8 with gonadal dysgenesis (GD), 3 with 3 beta-hydroxy-steroid dehydrogenase deficiency (3 beta HSD), 5 with androgen insensitivity syndrome (AIS), 4 with 5 alpha-reductase 2 (SRD5A2) deficiency, and 4 were of unknown origin or idiopathic, Our results showed that while testosterone was low and gonadotropins elevated in patients with either GD or 3 beta HSD, AMH was low in the former and high in the latter. Serum AMH and gonadotropins were normal or high in patients with 3 beta HSD or AIS, but these could be distinguished by testosterone levels. Serum testosterone and gonadotropins were normal or high in AIS and SRD5A2 deficiency patients; however, while AMH was elevated in AIS, it was not the case in SRD5A2 deficiency patients, indicating that testosterone is sufficient to inhibit AMH within the testis. In idiopathic cases gonadotropins and testosterone were normal, and AMH was normal or low. We conclude that the combined measurement of androgens, AMH and gonadotropins helps to establish the diagnosis in intersex patients.
- ItemAcesso aberto (Open Access)Resposta esteroidogênica induzida por hCG em garanhões jovens Mangalarga: testosterona e sulfato de estrona plasmáticos(Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo, 2000-01-01) Lima, Samira Barbosa; Wischral, Aurea; Ferreira, Fernando; Verreschi, Ieda Therezinha do Nascimento [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Leydig cell acute steroidogenic response, in seven young Mangalarga stallions (2.6 - 4.5 ys), was evaluated through human Corionic Gonadotrophin (hCG) stimulation. The animals were treated with 5,000 UI (hCG) Vetecor®, i.v., in bollus, at 8 a.m. Plasma samples were collected by venopuncture at 7 a.m. (basal), 4, 24, 48, and 72 hours after stimulation (from 28 to August 31). Testosterone (T) and estrone sulphate (E1SO4) was measured by radioimmunoassay (RIA), intra-assay variation <= 5%. Data were analysed by Friedman and Wilcoxon Matched-Pairs tests (p <= 0.05). The magnitude of hormonal production after stimulation was analysed by Percentual Variation (delta %). At 48 hours, 57.1% of the animals (n = 4) had maximal response (T) with values 5.7 greater than basal. And although 57.1% of them (n = 4) had maximal response (E1SO4) at 4 hours, the maximal value was 1.6 greater than basal at 24 hours. The hCG may be employed to stimulate the Leydig cell steroidogenic response and 5,000 UI is enough to stimulate testicular steroidogenesis. The estrogen basal concentrations are higher than testosterone basal concentrations. This protocol could be applied to identify fertility problems of endocrine origin in horses of this breed.