Navegando por Palavras-chave "Lesões Experimentais por Radiação"

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    Effects of ionizing radiation on bone neoformation: histometric study in Wistar rats tibiae
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-12-01) Lucatto, Susette Cavicchioli [UNIFESP]; Guilherme, Arnaldo [UNIFESP]; Dib, Luciano Lauria [UNIFESP]; Segreto, Helena Regina Comodo [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Gumieiro, Emne Hammoud [UNIFESP]; Jahn, Ricardo Schmitutz [UNIFESP]; Leite, Ronaldo Antonio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Paulista University Faculty of Dentistry Department of Stomatology
    PURPOSE: Comparing the ionizing radiation effects on bone neoformation of rats tibiae previously submitted to radiotherapy with a single dosage of 30Gy with the contralateral tibiae that have received secondary radiation. METHODS: In thirty male Wistar rats, 30 days before surgical procedure when round defects would be created on the bone, the right tibia was irradiated with 30Gy and the left tibia received a calculated secondary radiation dose of 7Gy. Sacrifices were performed after 4, 7, 14, 21, 56 and 84 postoperative days and both tibiae were removed for histological processing. RESULTS: The left tibiae that received the dose of 7Gy has shown more bone neoformation from 14th postoperative days, giving evidences of less damage to cellular population responsible by bone neoformation. On the other hand, the dose of 30Gyon right tibiae did not exhibit significant differences among the periods, suggesting damage of long-lasting or even permanent duration. CONCLUSION: Tibiae submitted to radiation dose of 30Gy have shown more damage to bone cells than tibiae that received secondary radiation dose of 7Gy, especially observed on 14th, 56th and 84th postoperative days.
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    Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2010-06-01) Gumieiro, Emne Hammoud [UNIFESP]; Abrahão, Márcio [UNIFESP]; Jahn, Ricardo Schmitutz [UNIFESP]; Segretto, Helena [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Nannmark, Ulf; Granström, Gösta; Dib, Luciano Lauria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Göteborg University Department of Anatomy and Cell Biology; The Sahlgrenska Academy of Göteborg University Institute for Clinical Sciences Department of Otolaryngology, Head and Neck Surgery; Paulista University Faculty of Dentistry Department of Stomatology
    PURPOSE: To evaluate the influence of PRP addition on bone repair of circular defects created in irradiated tibiae of rats by histometric analysis. METHODS: Sixty male Wistar rats had the right tibiae irradiated with 30 Gy. After 30 days monocortical defects were created and platelet-rich plasma was applied in 30 rats. In the control group defects were created but not filled. The animals were desanguinated after 4, 7, 14, 21, 56 and 84 days and the tibiae removed for histological processing. RESULTS: There was a tendency in the PRP group to increased bone neoformation from 14-days to 84-days; in the control group increased bone neoformation was not seen after 21 days or later. CONCLUSION: The addition of platelet-rich plasma had a beneficial effect in the initial cellular regeneration period and enhanced bone formation in later periods when compared to control.