Navegando por Palavras-chave "Laminin receptor"

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    Identification of enolase as a laminin-binding protein on the surface of Staphylococcus aureus
    (Elsevier B.V., 2004-05-01) Carneiro, Celia Regina Whitaker [UNIFESP]; Postol, Edilberto; Nomizo, Regina; Reis, Luiz Fernando Lima; Brentani, Ricardo Renzo; Hosp Canc AC Camargo; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Ludwig Inst Canc Res
    We have previously demonstrated that Staphylococcus aureus, a highly invasive bacteria, presents a 52-kDa surface protein that mediates its binding to laminin. in order to better characterize this receptor, we excised this putative laminin receptor from two-dimensional (2-D) PAGE and used it as antigen for raising a mouse hyperimmune serum which was for screening an S. aureus expression library. A single clone of 0.3 kb was obtained, and its sequence revealed 100% homology with S. aureus a-enolase. Moreover, amino acid sequencing of the 52-kDa protein eluted from the 2-D gel indicated its molecular homology with a-enolase, an enzyme that presents a high evolutionary conservation among species. in parallel, monoclonal antibodies raised against the S. aureus 52-kDa band also recognized yeast a-enolase in western blot analysis. These monoclonal antibodies were also able to promote capture of iodine-labeled bacteria when adsorbed to a solid phase, and this capture was inhibited by the addition of excess rabbit muscle a-enolase. Finally, the cell surface localization of S. aureus a-enolase was further confirmed by flow cytometry. Hence, a-enolase might play a critical role in the pathogenesis of S. aureus by allowing its adherence to laminin-containing extracellular matrix. (C) 2004 Elsevier SAS. All rights reserved.