Navegando por Palavras-chave "Kalanchoe pinnata"

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    Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)
    (Natural Products Inc, 2018) Pereira, Kassia M. F. [UNIFESP]; Grecco, Simone S.; Figueiredo, Carlos R. [UNIFESP]; Hosomi, Jorge K. [UNIFESP]; Nakamura, Mari U. [UNIFESP]; Lago, Joao Henrique G.
    Kalanchoe pinnata (Crassulaceae) is a medicinal plant distributed in several areas of the World. Their leaves have been used, in folk medicine, for anxiety and sleep disorders among other proposes. Chemically, this plant is composed by organic acids, flavonoids, bufadienolides, triterpenoids and some ubiquitous compounds. In this study, the fresh leaves of K. pinnata were extracted in an accelerated solvent extraction (ASE) system using H2O (cold and hot) as well as with EtOH:H2O 1:1. The obtained crude extracts were analyzed by HPLC-UV-MS. As a result, were identified organic acids and flavonoids, these being quercetin-3-O-alpha-L-arabinopyranosyl-(1 -> 2)-alpha-L-rhamnopyranoside, kaempferol-3-O-alpha-L-arabinopiranosyl-(1 -> 2)-alpha-L-rhamnopyranoside, quercitrin, and trans-p-coumaryl glutaric acid the main compounds in the studied extracts. Obtained results revealed the predominance of flavonoids in extracts prepared using hot H2O (70.26 +/- 2.19%) or EtOH:H2O 1:1 (74.65 +/- 5.39%) while a reduction in the amount of these compounds was observed in the extracts prepared using cold H2O (54.41 +/- 2.93%). Additionally, crude extracts from leaves of K. pinnata extracted with hot H2O and EtOH:H2O 1:1 showed in vitro cytotoxicity on five tested cancer cell lines: murine melanoma (B16F10), human melanoma (A2058), human cervical carcinoma (HeLa), human leukemia (HL-60), and human glioblastoma (U87-MG), with IC50 values in the range of 10.6 +/- 2.5 - 91.3 +/- 7.2 mu g/mL. On the other hand, the extract prepared using cold H2O displayed reduced potential. These crude extracts were also tested against human foreskin fibroblasts (non-tumorigenic cells) showing IC50 values ranging from 79.5 to 90.2 mu g/mL. In conclusion, our results showed the impact that different extraction methods have on the chemical composition and pharmacological activity, which are notable for the anticancer activity associated to flavonoids.
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    Identificação e sazonalidade dos constituintes de Kalanchoe pinnata (Lans) Pers. – planta com ação uterolítica e ansiolítica
    (Universidade Federal de São Paulo (UNIFESP), 2016-12-31) Pereira, Kassia Martins Fernandes [UNIFESP]; Nakamura, Mary Uchiyama [UNIFESP]; http://lattes.cnpq.br/4320107502315102; http://lattes.cnpq.br/2989381959575224; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The Kalanchoe pinnata (Crassulacean) plant has a huge growth potential. It is specially used within Anthroposophic Medicine, where it has indication for anxiety and sleep disorders and for preterm labor prevention. Brazil's public health ministry recommends for further research regarding plants, in order to extend the range of safe therapeutic options offered to public health users (SUS). Objectives: To trace the phytochemical profile, evaluate it's composition and seasonality within various extraction mediums and seasons. Methods: The K. pinnata leaves were harvested and subjected to extraction in cool water, hot water, water:ethanol, dried-methanol, concentrate juice (undiluted), during summer and during winter. The anthroposophic medication was also analysed. The samples were analysed via High Performance Liquid Chromatography and Mass Spectrometry. Results: The plant's constituents were identified as hydroxycinnamic acids and flavonoids. The main components appeared in the following concentration sequence: Quercetin 3-O-?-L-arabinopyranosyl-(1?2)-?-L-rhamnopyranoside, Coumarylglucaric acid and Quercitrin. The medication also displayed Quercetin 3-O-?-L-arabinopyranosyl-(1?2)-?-L-rhamnopyranoside. The samples contents fluctuated according to extraction method and harvest season. The most concentred samples were the water-ethanol ones; whereas the summer samples showed a thorough component concentration, the winter ones had a larger concentration of the main components. Bufadienolides were not found in any extract. Conclusion: The applied methodology was able to trace the plant's profile. Flavonoids were the most evident class; with larger concentration in higher temperatures, with plenty sunlight exposure. The flavonoids seem to be responsible for the effects observed in other studies and clinic practice; therefore, future research regarding this plant is recommended.