Navegando por Palavras-chave "Inflamação Pulmonar Aguda"

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    Estudo do efeito anti-inflamatório do probiótico lactobacillus rhamnosus na inflamação pulmonar aguda induzida por lipopolissacarídeo de Escherichia coli em camundongos C57Bl/6
    (Universidade Federal de São Paulo (UNIFESP), 2019-12-18) Olimpio, Fabiana Regina Da Silva [UNIFESP]; Carvalho, Flavio Aimbire Soares De [UNIFESP]; Casarini, Dulce Elena [UNIFESP]; http://lattes.cnpq.br/8216583445045638; http://lattes.cnpq.br/3459727518339745; Universidade Federal de São Paulo (UNIFESP)
    OBJECTIVE: To study the anti-inflammatory effect of probiotic Lactobacillus rhamnosus (Lr) on cellular infiltrate and secretion of inflammatory mediators in an experimental model of acute LPS-induced pulmonary inflammation in C57Bl/6 mice. METHODS: Male C57Bl/6 mice were divided into 4 experimental groups (n = 7 mice for experimental group) described below: control (animals received intranasal saline solution instillation), LPS (mice received intranasal instillation of LPS), Lr / 1day + LPS (mice received Lr gastric oro at the same time as intranasally LPS), Lr / 14 days + LPS (mice received Lr gastric oro 1 time daily for 14 days prior to intranasal LPS instillation), Lr / 1day(mice received Lr gastric oro), Lr / 14 days (mice received Lr gastric oro 1 time daily for 14 days). All mice were euthanized 24 hours after LPS administration. Cellular infiltrate in the lung was assessed from the number of cells in the bronchoalveolar lavage (BAL) and changes in lung tissue were quantified from pulmonary morphometry. Inflammatory mediators in BAL and NF-κB in the lung were measured by ELISA. mRNA expression for toll like receptor (TLR) -2 and -4 receptors, MMP-9 and MMP-12 metaloproteases, and NF-κB transcription factor was evaluated by PCR. RESULTS: Lr attenuated cell migration and modulated secretion of pro- and anti-inflammatory mediators. Moreover, Lr reduced mRNA of TLR2 and -4, MMP-9, MMP-12 and NF-κB. CONCLUSION: The protective effect of Lr targets modulation of inflammatory markers and inhibition of NF-κB, both directly related to acute lung inflammation.