Navegando por Palavras-chave "Immunodiffusion"
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- ItemSomente MetadadadosAntigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis(Springer, 2013-02-01) Cordeiro, Rossana de Aguiar; Nobre Patoilo, Kharla Rabelo; Praciano, Silviane Bandeira; Astete Medrano, Delia Jessica; Farias Marques, Francisca Jakelyne de; Soares Martins, Liline Maria; Eulalio, Kelsen Dantas; Deus Filho, Antonio de; Salmito Cavalvanti, Maria do Amparo; Bezerra Fechine, Maria Auxiliadora; Nogueira Brilhante, Raimunda Samia; Camargo, Zoilo Pires de [UNIFESP]; Gadelha Rocha, Marcos Fabio; Costa Sidrim, Jose Julio; Univ Fed Ceara; Univ Fed Piaui; Inst Doencas Tropicais Natan Portella; Univ Integracao Int Lusofonia Afrobrasileira; Universidade Federal de São Paulo (UNIFESP); Univ Estadual CearaSerologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. in this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. the highest reactivity in the ID tests was seen in the F0-90 antigen. in the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. in conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. the tests could improve serological diagnosis of coccidioidomycosis in South America.
- ItemSomente MetadadadosEstudo comparativo entre as técnicas de imunodifusão radial e dupla difusão em gel de agar na dosagem das imunoglobulinas IgA e IgM no sangue de cordão umbilical(Universidade Federal de São Paulo (UNIFESP), 1984) Weckx, Lily Yin [UNIFESP]; Schmidt, Benjamin José [UNIFESP]
- ItemAcesso aberto (Open Access)Importância dos testes sorológicos para o diagnóstico de paracoccidioidomicose por paracoccidioides lutzii(Universidade Federal de São Paulo (UNIFESP), 2018-07-26) Angelo, Patricia Del [UNIFESP]; Camargo, Zoilo Pires de [UNIFESP]Paracoccidioidomycosis (PCM) is an endemic systemic mycosis, caused by the thermodymorphic fungus Paracoccidioides spp., being of great interest to the countries of Latin America, mainly to Brazil. Two different species have been identified: Paracoccidioides brasiliensis (with three cryptic species S1, PS2 and PS3) and Paracoccidioides lutzii. It presents at 25ºC in filamentous phase and at 37ºC in yeast phase, its dimorphism seems to be directly linked to the virulence. PCM is a fungal infection that can lead the individual to various sequelae and even death. For this reason it is very important the diagnosis to initiate the appropriate therapy cure of the patient. Serological tests are essential indirect methods used in the diagnosis and prognosis of the disease, in which specific antigens and antibodies are screened in the patient's serum. In order to increase the specificity and sensitivity of the serological tests it is advisable to use two or more serological tests for a definitive diagnosis. Among the techniques used, doubleagarose gel (ID), easy to handle and run immunodiffusion and conventional enzyme immunoassay (ELISA) are the most commonly used in routine diagnostic laboratories. In this investigation we intend to show the importance of the serological tests for the diagnosis of PCM, through two different methodologies, Immunodiffusion and Elisa with the intention of verifying the feasibility of the techniques used for the detection of antiP antibodies. lutzii in the sera of PCM patients by P. lutzii using complex antigenic preparations.
- ItemSomente MetadadadosSerologic Detection of Coccidioidomycosis Antibodies in Northeast Brazil(Springer, 2009-04-01) Cordeiro, Rossana de Aguiar; Bezerra Fechine, Maria Auxiliadora; Nogueira Brilhante, Raimunda Samia; Gadelha Rocha, Marcos Fabio; Freire da Costa, Ana Karoline; Tiemi Dias Nagao, Maria Aparecida; Camargo, Zoilo Pires de [UNIFESP]; Costa Sidrim, Jose Julio; Univ Fed Ceara; Univ Estadual Ceara; Universidade Federal de São Paulo (UNIFESP)Coccidioidomycosis is a systemic infection caused by Coccidioides spp. the disease is endemic in Brazil but its incidence is underreported as it is not a notifiable disease. This article presents the results of a serologic survey carried out with 229 volunteers in northeast Brazil by the immunodiffusion (ID) test with commercial Coccidioides spp. antigens. the commercial ID test detected 15 individuals without clinical diagnosis of the disease and two individuals in treatment for coccidioidomycosis. Regarding the epidemiological data, most of the positive individuals were male, between 18 and 65 years of age and were engaged in armadillo hunting. Three women who had never participated in armadillo hunts also displayed positive results for coccidioidal antibodies. Besides armadillo hunts, exposure to environmental dust in endemic areas may account for the serologic response detected in the study. the data from this study suggest the importance of performing epidemiological surveys for coccidioidomycosis in order to understand the prevalence of this disease in Brazil.