Navegando por Palavras-chave "Hyperlipidic Diet"
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- ItemAcesso aberto (Open Access)Avaliação do potencial pró-inflamatório induzido por Lipopolissacarídeo, combinado ou não com o Ácido Graxo Saturado Palmitato, em linhagem celular de micróglias: estudo in vitro da relação obesidade, inflamação e sistema nervoso central(Universidade Federal de São Paulo, 2021-02-25) Silva, Valkiria Guilherme Assis da [UNIFESP]; Mendes-da-Silva, Cristiano [UNIFESP]; Cheberle, Ana Isabel do Prado [UNIFESP]; http://lattes.cnpq.br/9688543931584508; http://lattes.cnpq.br/7868915353525184; http://lattes.cnpq.br/0905802370100856; Universidade Federal de São Paulo (UNIFESP)Introdução: A obesidade e a dieta hiperlipídica (DHL) contribuem para um estado pró-inflamatório no sistema nervoso central (SNC) pela ativação da cascata de sinalização do receptor toll-like 4 (TLR-4) através da ligação com lipopolissacarídeo (LPS) e ácido graxo saturado palmitato (PALM), que induzem a produção de citocinas pró-inflamatórias, podendo gerar alterações metabólicas, dano e morte celular. Objetivo: Avaliar a resposta inflamatória das culturas de células BV-2 (micróglias) expostas ao LPS ou PALM ou a combinação de ambos. Metodologia: As células foram cultivadas em meio DMEM contendo 10% de Soro Fetal Bovino (SFB) a 37°C, sob atmosfera de 5% de CO₂. Foi realizado o teste de MTT com diferentes dosagens de LPS e PALM, e o tratamento celular com esses componentes combinados ou isolados. O sobrenadante foi utilizado na técnica ELISA para quantificar a citocina TNF-alfa. Foi avaliada a expressão de JNK e pJNK do extrato protéico das células através de Western Blotting. Resultados: PALM e LPS combinados e isolados diminuíram a viabilidade celular. PALM não causou alterações nos parâmetros inflamatórios avaliados. LPS gerou uma resposta pró-inflamatória, apresentando níveis aumentados de TNF-alfa e pJNK. Considerações Finais: Ambos tratamentos diminuíram a viabilidade celular, mas somente LPS promoveu um ambiente pró-inflamatório. Os resultados sugerem que a combinação de PALM e LPS pode produzir um estímulo pró-inflamatório ainda mais potente com capacidade de danificar o tecido nervoso, mimetizando in vitro, possíveis efeitos da DHL sobre o SNC.
- ItemSomente MetadadadosEfeito Da Chia ( Salvia Hispanica L) Sobre Parâmetros Metabólicos E Inflamatórios No Diabetes Mellitus, Em Camundongos(Universidade Federal de São Paulo (UNIFESP), 2017-11-30) Oliveira, Cinthia Romeika De [UNIFESP]; Silveira, Vera Lucia Flor [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction and Objectives: Diabetes mellitus (DM) is a chronic disease, characterized by hyperglycemia, which may be caused by the absence or deficient of insulin secretion or peripheral resistance to this hormone. As a consequence of this alteration, the adipose tissue of individuals with type 2 DM (DM2), presents high rates of lipolysis, aggravating the existing insulin resistance. Several studies have shown the importance of omega-3 polyunsaturated fatty acids (PUFAs w-3) as complementary therapy in the treatment of chronic diseases, including DM. Studies have shown that these PUFA decrease the inflammatory process and may improve insulin resistance. chia seeds, which has the vegetable oil with the highest concentration of PUFAs w-3, known until now, has been widely used by the population for reduction of weight, cholesterol and triglyceride, and also prevention of diabetes and cardiovascular diseases. However, the scientific papers which study these relations are few and still quite contradictory. This study aims to analyze the preventive effect of dietary supplementation with chia oil on metabolic and inflammatory parameters in mice submitted to the DM2 model, induced by hyperlipidic diet and streptozotocin (STZ). For this, we used male C57bl / 6 mice, divided into three groups: control (C), diabetic (D) and diabetic treated with chia (DC). The animals in group C received a normolipidic diet for 6 weeks and the animals in groups D and DC received a hyperlipidic diet and daily doses of water (D) or chia oil (DC). At the end of the third week of feeding, animals in groups D and DC were induced to partial insulin deficiency by 1 dose (45mg / kg) of STZ during 5 consecutive days. At the end of the sixth week the mice were euthanized, blood, liver and adipose tissues removed. Results and Conclusions: Preventive treatment with chia oil promoted beneficial effects such as decrease body mass gain without modifying caloric and energy consumption, reduction of visceral fat, lipolysis and adipocyte volume of the epididymal tissue and increase of HDL- c. However, although this treatment partially improved glucose tolerance, it did not improve insulin resistance. It appears that the beneficial effects induced by chia in lipid metabolism were not sufficient for the improvement of insulin resistance required for the treatment of DM2.
- ItemSomente MetadadadosEfeito da ingestão da farinha da semente de chia (Salvia Hispanica L) no cólon intestinal de camundongos obesos(Universidade Federal de São Paulo (UNIFESP), 2017-08-31) Miranda, Danielle Araujo de [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Obesity has been considered a public health issue worldwide, in which the main causes are the intake of a hypercaloric diet as well as sedentarism. It is often associated with with several other diseases such as insulin resistance, type 2 diabetes mellitus, non-alcoholic fatty liver disease and cardiovascular diseases. Increased intestinal permeability may favor endotoxemia generated by lipopolysaccharide, a substance present in the cell membrane of Gram-negative bacteria, and consequently an increase in systemic inflammation and metabolic diseases. On the other hand, the consumption of a healthy diet can help in the prevention and treatment of the metabolic syndrome. In this way, the chia seed (Salvia hispánica L.), rich in polyunsaturated fatty acids, may present an anti-inflammatory role. In addition, chia is rich in antioxidants like caffeic acid and gallic acid, fiber and minerals. However, there are few studies involving chia seed, inflammatory mechanisms and intestinal permeability. Thus, the objective of this study was to analyze the effects of chia administration on intestinal permeability in obese mice. Swiss mice were treated with a hyperlipidic diet associated or not with supplementation with 3% chia flour for 16 weeks. We analyzed the metabolic profile, such as serum triglyceride, cholesterol, HDL-cholesterol, glucose tolerance test, antioxidant enzyme activity and the quantification of inflammatory pathways and tight junctions of the intestinal colon. The results show that a hyperlipidic diet intake promoted an increase of the mesenteric white adipose tissue weight and in glucose intolerance, and a decrease in the activity of hepatic catalase and the amount of occludin in the intestinal colon. Supplementation with 3% of chia flour was not efficient in decreasing the deleterious effects of lipid-rich dietary intake. In addition, supplementation with chia worsened glucose intolerance in animals receiving a hyperlipidic diet whereas in animals receiving a control diet, supplementation decreased the amount of occludin in the intestinal colon. In conclusion, the beneficial effects of chia seems to be dependent of the quantity how It is offered, since our data are conflicting with those of the literature, however, it is important to note that most of the studies, unlike our protocol, used chia in the form of seed or oil, and not the flour.
- ItemSomente MetadadadosEstudo do papel da proteína GCN1 na gênese de doenças metabólicas em camundongos(Universidade Federal de São Paulo (UNIFESP), 2019-04-25) Guerra, Beatriz Alves [UNIFESP]; Mori, Marcelo Alves Da Silva [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)To study the importance of GCN1 in the adipose tissue of mice for the genesis of metabolic diseases in response to different nutritional interventions. Methods: We performed crosses to obtain the following three strains: iMyf5Cre