Navegando por Palavras-chave "Human dental stem cells"

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    Cytogenetic instability of dental pulp stem cell lines
    (Springer, 2012-02-01) Duailibi, Monica Talarico [UNIFESP]; Kulikowski, Leslie Domenici; Duailibi, Silvio Eduardo [UNIFESP]; Nunes Lipay, Monica Vannucci [UNIFESP]; Melaragno, Maria Isabel; Ferreira, Lydia Masako [UNIFESP]; Vacanti, Joseph Phillip; Yelick, Pamela Crotty; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); INCT Biofabris; Massachusetts Gen Hosp; Harvard Univ; Dept Surg; Tufts Univ
    Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. the heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.
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    Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development
    (Wiley-Blackwell, 2011-07-01) Duailibi, Monica Talarico [UNIFESP]; Duailibi, Silvio Eduardo [UNIFESP]; Duailibi Neto, Eduardo Felippe; Negreiros, Renata Matalon; Jorge, Waldyr Antonio; Ferreira, Lydia Masako [UNIFESP]; Vacanti, Joseph Phillip; Yelick, Pamela Crotty; Tufts Univ; Universidade Federal de São Paulo (UNIFESP); Natl Inst Sci & Technol; Universidade de São Paulo (USP); Massachusetts Gen Hosp; Dept Surg; Harvard Univ
    Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). the greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.