Navegando por Palavras-chave "Hormônio luteinizante"

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    Avaliação do desenvolvimento pubertário masculino normal através da correlação entre dados clínicos antropométricos e laboratoriais como o hormônio luteinizante, a testosterona e o antígeno prostático específico
    (Universidade Federal de São Paulo (UNIFESP), 1994) Arrais, Ricardo Fernando [UNIFESP]; Verreschi, Ieda Therezinha do Nascimento [UNIFESP]
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    Correlação entre os valores do pico de Lh, ultra-sonografia transvaginal e datação do endométrio no diagnóstico da ovulação
    (Universidade Federal de São Paulo (UNIFESP), 1998) Samama, Marise [UNIFESP]
    No Setor de Reproducao Humana da Disciplina de Ginecologia da Escola Paulista de Medicina da Universidade Federal de São Paulo, realizou-se um estudo com 40 pacientes inferteis, com o objetivo de se estudar a correlacao entre tres diferentes metodos diagnosticos de ovulacao: dosagem seriada plasmatica de LH, ultra-sonografia transvaginal e datacao histologica do endometrio. Encontrou-se uma elevada e significante correlacao entre os tres metodos estudados. Esta correlacao foi de 98 por cento entre o pico de LH e a ultra-sonogracia transvaginal, 97 por cento entre o pico de LH e a datacao do endometrio, e entre a ultra-sonografia transvaginal seriada e a datacao histologica do endometrio foi de 98 por cento . Houve, tambem, diferenca significante na analise simultanea entre eles, mostrando que o pico da gonadotrofina tende a determinar a ovulacao um dia antes dos outros dois metodos. Nao houve diferenca signifjcante entre a ultra-sonografia transvaginal e a datacao do endometrio. Analisou-se, ainda, as discordancias entre os metodos. Diferencas de ate dois dias foram observadas, o que sugere a importancia de se associarem metodos para o diagnostico de ovulacao
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    Efeito da adição do LH durante o estímulo ovariano e maturação oocitária no perfil lipídico de oócitos murinos
    (Universidade Federal de São Paulo (UNIFESP), 2016-02-02) Oleinki, Talitha Dinardo [UNIFESP]; Fraietta, Renato [UNIFESP]; http://lattes.cnpq.br/1545035937368744; http://lattes.cnpq.br/7699135183138091; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The addition of LH in assisted reproduction aiming to obtain a higher number of viable follicles for the treatment is still controversial. A good embryonic development depends on the follicular growth and proper oocyte maturation. Because of that, the association between alkaline comet assay and the study of oocyte lipidomics may help to understand the biological processes involved in oocyte maturation and the influence of hormonal protocol. This study may contribute in the future with effective hormonal protocols, improving the quality of oocytes and pregnancy rates. Objective: Evaluate the effect of adding LH on DNA integrity of cumulus cells and on lipid profile of murine oocytes. Methods: Female mice C57BL / 6J 23 to 28 days old received three different stimulation protocols intraperitoneally (i.p.): (i) only PMSG; (ii) PMSG plus hCG (iii) PMSG plus LHr. After 48 hours of administration, immature oocytes were collected and cultured for 24 hours in three different culture medium for maturation: (i) only FSH; (ii) FSH plus hCG (iii) FSH plus LHr. After this period, the oocytes which had the first polar body were frozen at -80oC until the analysis by electrospray ionization (ESI) and cumulus cells were subjected to alkaline comet technique to measure the integrity of DNA. The principal component analysis and discriminant analysis by least squares were performed and 35 ions with greater representation were identified by the variable importance in the projection. Results: There was no statistical difference in the maturation rate and DNA fragmentation of cumulus cells in different groups analyzed. The fingerprinting analysis of the lipid profile of oocytes matured in vitro identified twenty lipids. The hyper-represented lipids in the group stimulated with the addition of hCG and matured in culture medium only with FSH (HF group) are: phosphatidylethanolamine, polyketide (flavonoid), sterol, phosphatidylserine, polyketide (ansamycin), sphingomyelin and phosphatidylcholine; in the group stimulated and matured in the presence of hCG (HH group) phosphatidylglycerol, phosphatidylserine, sphingolipids and triacylglycerol are hyper-represented; and in group stimulated with addition of LHr and matured in culture medium only with FSH (LF group) the polyketide (flavonoid) and sphingolipids are hyper-represented. Conclusion: The protocol used for hormonal stimulation and MIV modify the lipid profile of the oocyte, but does not alter the oocyte maturation rate and the DNA integrity of cumulus cells.