Navegando por Palavras-chave "Hoechst-33258"

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    Ionic and biochemical characterization of bovine intervertebral disk
    (Taylor & francis inc, 2016) Martins, Delio Eulalio [UNIFESP]; Medeiros, Valquiria Pereira; Demerov, Gabriela Feitosa [UNIFESP]; Accardo, Camila Melo [UNIFESP]; Paredes-Gamero, Edgar Julian [UNIFESP]; Wajchenberg, Marcelo [UNIFESP]; Reginato, Rejane Daniele [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Puertas, Eduardo Barros [UNIFESP]; Faloppa, Flavio [UNIFESP]
    Introduction: Intervertebral disks have been associated with low back pain, and many therapies have been proposed for its treatment. The cellular and molecular knowledge of intervertebral disks composition and precise methods to quantify disk components are important for any type of proposed therapy. Thus, the aim of this study was to correlate glycosaminoglycans presence with the quantitation of cells, ions and collagen fiber distributions in different intervertebral disk sections. Methods: In total, 14 intervertebral disks were used from cattle. All of the disks were dehydrated, separated in seven sections and digested in sodium-free papain buffer. Glycosaminoglycan measurements were performed in the samples according to agarose electrophoresis method