Navegando por Palavras-chave "Hepatic lipase gene"

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    Análise do polimorfismo C>514T do gene da Lípase Hepática em mulheres sob reposição estrogênica
    (Universidade Federal de São Paulo (UNIFESP), 2012-02-01) Pulchinelli Júnior, Alvaro [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background: Hepatic lipase (HL), an enzyme present in the hepatic sinusoids, is responsible for the lipolysis of lipoproteins. Human HL contains four polymorphic sites: G-250A, T-710C, A-763G, and C-514T single-nucleotide polymorphism (SNPs). The last polymorphism is the focus of the current study. The genotypes associated with the C-514T polymorphism are CC (normal homozygous – W), CT (heterozygous – H), and TT (minor-allele homozygous – M). HL activity is significantly impaired in individuals of the TT and CT genotypes. A total of 58 postmenopausal women were studied. The subjects were hysterectomized women receiving hormone replacement therapy consisting of 0.625 mg of conjugated equine estrogen once a day. The inclusion criteria were menopause of up to three years and normal blood tests, radiographs, cervical-vaginal cytology, and densitometry. DNA was extracted from the buccal and blood cells of all 58 patients using a commercially available kit (GFX® - Amersham-Pharmacia, USA). Results: Statistically significant reductions in triglycerides (t=2.16; n=58; p=0.03) but not in total cholesterol (t=0.14; n=58; p=0.89) were found after treatment. This group of good responders were carriers of the T allele; the CT and TT genotypes were present significantly more frequently than in the group of non-responders (p=0.02 or p=0.07, respectively). However, no significant difference in HDL-C (t=0.94; n=58; p=0.35) or LDL-C (t=- 0.83; n=58; p=0.41) was found in these patients. Conclusions: The variation in lipid profile associated with the C-514T polymorphism is significant, and the T allele is associated with the best response to ERT.
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    Positive association of the hepatic lipase gene polymorphism c.514C > T with estrogen replacement therapy response
    (Biomed Central Ltd, 2011-11-02) Pulchinelli, Alvaro [UNIFESP]; Costa, Ana Maria Massad [UNIFESP]; Carvalho, Cristina Valletta de [UNIFESP]; Nogueira-de-Souza, Naiara Corrêa [UNIFESP]; Haidar, Mauro Abi [UNIFESP]; Andriolo, Adagmar [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background: Hepatic lipase (HL), an enzyme present in the hepatic sinusoids, is responsible for the lipolysis of lipoproteins. Human HL contains four polymorphic sites: G-250A, T-710C, A-763G, and C-514T single-nucleotide polymorphism (SNPs). the last polymorphism is the focus of the current study. the genotypes associated with the C-514T polymorphism are CC (normal homozygous - W), CT (heterozygous - H), and TT (minor-allele homozygous - M). HL activity is significantly impaired in individuals of the TT and CT genotypes. A total of 58 post-menopausal women were studied. the subjects were hysterectomized women receiving hormone replacement therapy consisting of 0.625 mg of conjugated equine estrogen once a day. the inclusion criteria were menopause of up to three years and normal blood tests, radiographs, cervical-vaginal cytology, and densitometry. DNA was extracted from the buccal and blood cells of all 58 patients using a commercially available kit (GFX (R) - Amersham-Pharmacia, USA).Results: Statistically significant reductions in triglycerides (t = 2.16; n = 58; p = 0.03) but not in total cholesterol (t = 0.14; n = 58; p = 0.89) were found after treatment. This group of good responders were carriers of the T allele; the CT and TT genotypes were present significantly more frequently than in the group of non-responders (p = 0.02 or p = 0.07, respectively). However, no significant difference in HDL-C (t = 0.94; n = 58; p = 0.35) or LDL-C (t = -0.83; n = 58; p = 0.41) was found in these patients.Conclusions: the variation in lipid profile associated with the C-514T polymorphism is significant, and the T allele is associated with the best response to ERT.