Navegando por Palavras-chave "Franz cell"

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    Desenvolvimento de nanopartículas lipídicas sólidas contendo filtro solar orgânico e óleo vegetal da flora brasileira
    (Universidade Federal de São Paulo, 2017-06-23) Bim, Antonio Vinicius Kosiski [UNIFESP]; Silva, Vânia Rodrigues Leite e [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The use of sunscreen products is widely promoted by schools, government agencies and health-related organizations to minimize sunburn and skin damage, which can lead to skin cancer. In this study, we developed stable solid lipid nanoparticles (SLN) containing the UV chemical filter octyl methoxycinnamate (OMC), associated or not with wheat germ oils (Triticum sativum Lank) and urucum oil (Bixa orellana L.) using emulsification techniques, high shear dispersion and high pressure homogenization. It was possible to obtain nanoparticles with adequate dimensions and stability for application in sunscreen formulations. Ex vivo tests (skin diffusion) did not show significant permeation, whereas in vitro tests (PAMPA) demonstrated that SLNs are able to diffuse through the lipid layer without altering its size, suggesting stability in the skin. In vitro solar protection factor (SPF) studies indicate that when 20% of the OMC was replaced by urucum oil, there was no reduction in SPF, suggesting that a decrease in the amount of chemical filter may be a viable alternative to an effective sunscreen, in combination with oils as urucum, rich in antioxidants. The results corroborate a strong trend to increase the safety of sun protection products by reducing the use of ultraviolate (UV) chemical filters producing formulations with lower concentrations of OMC while maintaining SPF. Further investigations of in vivo SPF are required to evaluate the suitability of these formulations for human use.
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    Development and Evaluation of Lipid Nanoparticles Containing Natural Botanical Oil for Sun Protection: Characterization and in vitro and in vivo Human Skin Permeation and Toxicity
    (Karger, 2018) Andreo-Filho, Newton [UNIFESP]; Kosiski Bim, Antonio Vinicius [UNIFESP]; Kaneko, Telma Mary; Kitice, Nidia Ayumi [UNIFESP]; Haridass, Isha N.; Abd, Eman; Lopes, Patricia Santos [UNIFESP]; Thakur, Sachin S.; Parekh, Harendra S.; Roberts, Michael S.; Grice, Jeffrey E.; Benson, Heather A. E.; Leite-Silva, Vania Rodrigues [UNIFESP]
    The use of sunscreen products is widely promoted by schools, government agencies, and health-related organizations to minimize sunburn and skin damage. In this study, we developed stable solid lipid nanoparticles (SLNs) containing the chemical UV filter octyl methoxycinnamate (OMC). In parallel, we produced similar stable SLNs in which 20% of the OMC content was replaced by the botanical urucum oil. When these SLNs were applied to the skin of human volunteers, no changes in fluorescence lifetimes or redox ratios of the endogenous skin fluorophores were seen, suggesting that the formulations did not induce toxic responses in the skin. Ex vivo (skin diffusion) tests showed no significant penetration. In vitro studies showed that when 20% of the OMC was replaced by urucum oil, there was no reduction in skin protection factor (SPF), suggesting that a decrease in the amount of chemical filter may be a viable alternative for an effective sunscreen, in combination with an antioxidant-rich vegetable oil, such as urucum. There is a strong trend towards increasing safety of sun protection products through reduction in the use of chemical UV filters. This work supports this approach by producing formulations with lower concentrations of OMC, while maintaining the SPF. Further investigations of SPF in vivo are needed to assess the suitability of these formulations for human use. (C) 2017 S. Karger AG, Basel