Navegando por Palavras-chave "Filamentous Fungi"

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    Avaliação de diferentes formulações com potencial clareador, alteração de cor, rugosidade e microdureza em esmalte dental bovino
    (Universidade Federal de São Paulo (UNIFESP), 2019-11-28) Pauli, Maria Cibelle [UNIFESP]; Leonardi, Gislaine Ricci [UNIFESP]; http://lattes.cnpq.br/4127354753135118; http://lattes.cnpq.br/0039354511931660; http://lattes.cnpq.br/0039354511931660; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate in vitro the effectiveness of fungal formulations (edible mushrooms and Penicillium crustosum Thom fungus isolated from molded coffee capsule) in tooth whitening, initially using coffee beverage whitening as a reference, followed by the application of these block formulations dental cattle. Methodology: The evaluation of the whitening effectiveness was given by comparing the results obtained with conventional methods, evaluating the color after the treatments (ΔL *, Δa *, Δb * and ΔE), besides the dental structure evaluation, using roughness variation (ΔRa ), surface transverse microhardness (CSMH) and scanning electron microscopy (SEM) images as a parameter. Statistical analysis was performed using one-way analysis of variance (ANOVA) and Tukey test for microhardness and roughness results (a = 0.05). The color data did not meet the assumptions of a parametric analysis and was analyzed by the non-parametric Wilcoxon test in the analyzes between the final and initial times (a = 0.05). The color data did not meet the assumptions of a parametric analysis and were analyzed by the nonparametric Wilcoxon in the analyzes between the end times and initial (a = 0.05). Results: The previous results of the effectiveness of specific enzymes in the discoloration of coffee drink and in the bleaching of bovine dental blocks showed that both mushrooms and the fermentation extract with the filamentous fungus were able to clear the dental blocks, with ΔE values of approximately ¾ of the value obtained with conventional bleaches. Conclusion: It is concluded that the use of fungi as an alternative method in tooth whitening is promising, since it consists of a natural method and may cause fewer adverse reactions when compared with conventional methods.
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    Purification and biochemical characterization of an extracellular serine peptidase from aspergillus terreus
    (Taylor & francis inc, 2016) Biaggio, Rafael Tage; da Silva, Ronivaldo Rodrigues; da Rosa, Nathalia Gonsales; Ribeiro Leite, Rodrigo Simoes; Arantes, Eliane Candiani; de Freitas Cabral, Tatiana Pereira; Juliano, Maria A. [UNIFESP]; Juliano, Luiz [UNIFESP]; Cabral, Hamilton
    Peptidases are important because they play a central role in pharmaceutical, food, environmental, and other industrial processes. A serine peptidase from Aspergillus terreus was isolated after two chromatography steps that showed a yield of 15.5%. Its molecular mass was determined to be 43 kD, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This peptidase was active between pH 5.0 to 8.0 and had maximum activity at pH 7.0, at 45 degrees C. When exposited with 1 M of urea, the enzyme maintained 100% activity and used azocasein as substrate. The N-terminal (first 15 residues) showed 33% identity with the serine peptidase of Aspergillus clavatus ES1. The kinetics assays showed that subsite S-2 did not bind polar basic amino acids (His and Arg) nonpolar acidic amino acids (Asp and Glu). The subsite S-1 showed higher catalytic efficiency than the S-2 and S-3 subsites.