Navegando por Palavras-chave "Fibroblast growth factor"

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    Estudo comparativo da ação do fator de crescimento de fibroblastos e fragmentos de nervo na regeneração de nervo tibial em ratos
    (Sociedade Brasileira de Ortopedia e Traumatologia, 2007-01-01) Hirakawa, Celso Kiyoshi [UNIFESP]; Grecco, Marco Aurélio Sertório [UNIFESP]; Santos, João Baptista Gomes dos [UNIFESP]; Leite, Vilnei Matiolli [UNIFESP]; Faloppa, Flávio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    OBJECTIVE: To quantitatively compare rats' tibial nerve regeneration stimulation by Fibroblast Growth Factor (FGF) and nerve fragments using silicone tubes. METHODS: 18 Wistar rats were employed in this experiment. The experimental surgery consisted of resection of an 8-mm tibial nerve segment, followed by an interposition of silicone tubes. On the right side, the tube was filled with a Fibroblast Growth Factor (FGF) solution, and on the left side, it was filled with 1 mm nerve segments. After three months, the animals were submitted to an additional surgery for exposing tibial nerves to the neuronal marker Fluoro-Gold®. After 48 hours, they were perfused with a paraformaldehyde solution and the medullar segment between L3 and S1 was removed and cut into 40 mum-thick segments. RESULTS AND CONCLUSION: The results of neuronal counts showed a higher amount of neurons on the side where FGF was used compared to the side where nervous fragments were placed, suggesting a superior performance of the fibroblast growth factor over nerve fragments for stimulating nervous regeneration in silicone tubes.
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    Fish and omega-3 fatty acid intake in relation to circulating fibroblast growth factor 23 levels in renal transplant recipients
    (Elsevier B.V., 2014-12-01) Baia, L. C. [UNIFESP]; Van den Berg, E.; Vervloet, M. G.; Heilberg, I. P. [UNIFESP]; Navis, G.; Bakker, S. J. L.; Geleijnse, J. M.; Kromhout, D.; Soedamah-Muthu, S. S.; De Borst, M. H.; NIGRAM Consortium; Univ Groningen; Universidade Federal de São Paulo (UNIFESP); VU Univ Med Ctr Amsterdam; Wageningen Univ
    Background and aims: A high circulating fibroblast growth factor 23 (FGF23) level is an independent risk factor for cardiovascular mortality in renal transplant recipients and the general population. N-3 fatty acids eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) may contribute to cardiovascular risk reduction. We investigated whether fish and EPA-DHA intake are related to FGF23 levels in renal transplant recipients.Methods and results: We performed a cross-sectional analysis in 619 stable renal transplant recipients (mean age 53 years, 57% male, estimated glomerular filtration rate [eGFR] 53 +/- 20 mL/min/1.73 m(2)). Dietary intake was assessed by a 177-item food frequency questionnaire. Serum intact FGF23 was measured by ELISA. We examined differences in FGF23 levels across categories of fish and EPA-DHA intake using analysis of variance models adjusted for age, sex, dietary and lifestyle factors and key determinants of FGF23. Patients consumed on average 15 g of fish and 139 mg EPA-DHA/day. Median FGF23 was 62 pg/mL (IQR 43-98 pg/mL). Higher dietary EPA-DHA and fish intake were associated with lower serum FGF23 levels. Subgroup analyses revealed that particularly in patients with reduced renal function (eGFR <60 mL/min/1.73 m(2)), adjusted FGF23 levels (114, 79, 75 pg/mL, P = 0.0001) were inversely associated with tertiles of EPA-DHA intake. Similarly, we observed an inverse association between fish consumption and serum FGF23 levels in adjusted analyses.Conclusion: A higher intake of fish and dietary n-3 fatty acids (EPA-DHA) is related to lower circulating FGF23 levels in renal transplant recipients. Further research is needed to assess the causality of this association and the clinical implications. (C) 2014 Elsevier B.V. All rights reserved.
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    Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine
    (Springer London Ltd, 2017) Siqueira das Neves, Lais Mara; Marcondes Ferreira Leite, Gabriella de Paula; Marcolino, Alexandre Marcio; Pinfildi, Carlos Eduardo [UNIFESP]; Garcia, Sergio Britto; de Araujo, Joao Eduardo; de Oliveira Guirro, Elaine Caldeira
    The aim of this study was to investigate the effect of laser photobiomodulation (PBM) on the viability of the transverse rectus abdominis musculocutaneous (TRAM) flap in rats subjected to the action of nicotine. We evaluated 60 albino Wistar rats, divided into six groups of ten animals. Group 1 (saline) underwent the surgical technique to obtain a TRAM flap