Navegando por Palavras-chave "Facial Nerve"
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- ItemSomente MetadadadosEstudo Histológico Da Expressão Da Aquaporina 2 Em Nervo Facial De Ratos(Universidade Federal de São Paulo (UNIFESP), 2017-04-27) Gondra, Luiza De Almeida [UNIFESP]; Testa, Jose Ricardo Gurgel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: Facial palsy is a common mononeuropathy that occuring when interruption of facial nerve activity. The pathophysiology of idiopathic facial paralysis is uncertain and one of the possibilities would be the expression of aquaporins (AQPs), which influences neural edema and synaptic transmission by mechanisms of water homeostasis. Three AQPs (1, 2 and 4) were identified in the peripheral nervous system (PNS), and specifically in the facial nerve was identified AQP 1 and 2. The aim of the study was to describe the expression and histological location of AQP 2 in the facial nerve of rats. Methods: Five adult Wistar rats were used in the study. On the day zero (D0), four animals were submitted to compression of the extratemporal trunk of the right facial nerve (LESION group) and in one rat submitted to skin and subcutaneous dissection at right retroauricular region (SHAM group). On the following day (D1), the extratemporal segment (about 3 to 8 mm) of the right and left facial nerve of all animals were removed. The ten facial nerves samples were processed for paraffin sections to hematoxylin-eosin staining (HE) and immunohistochemistry using the anti-AQP 2 antibody. Qualitative analysis of the samples and Schwann cell immunolabelled counts where proceed. Results: Wallerian degeneration was observed in HE slides in 40% of the samples. The expression of AQP 2 on the facial nerve of rats was confirmed. In all nerves the presence of the antibody was observed, with greater expression in Schwann cells and endothelial cells and less expression in the cytoplasm of axons, perineural cells and epineurium. Schwann cell count revealed in two animals a statistical difference between the LESION group and the corresponding control nerves, with higher expression of AQP 2 on the nerves submitted to compression. Conclusion: The presence of AQP 2 in the facial nerve of rats was confirmed and the expression in this tissue was independent of any surgical intervention. After compression of the facial nerve, the marking of this protein increased, with possible mediation in the installation of the edema in this tissue.
- ItemAcesso aberto (Open Access)Facial nerve identification with fluorescent dye in rats(Acta Cirurgica Brasileira, 2016) de Melo, Giulianno Molina [UNIFESP]; Cervantes, Onivaldo [UNIFESP]; Covolan, Luciene [UNIFESP]; Baptista, Heloisa Allegro [UNIFESP]; Ferreira, Elenn Soares [UNIFESP]; Abrahao, Marcio [UNIFESP]PURPOSE: The parotidectomy technique still has an elevated paresis and paralysis index, lowering patient life's quality. The correct identification of the facial nerve can prevent nerve damage. Fluorescent dye identifies nerves in experimental studies but only few articles focused its use on facial nerve study in parotidectomies. We aimed to stain the rat facial nerve with fluorescent dye to facilitate visualization and dissection in order to prevent injuries. METHODS: Forty adult male Wistar rats were submitted to facial injection of saline solution (Gsf-control group, 10) or fluorescent dye solution (Gdye group, 30) followed by parotidectomy preserving the facial nerve, measuring the time for localization and facility of localization (LocTime and LFN). Nerve function was assessed using the Vibrissae Movements (PMV) and Eyelid Closure Motion (PFP) scores. RESULTS: Nerve localization was faster in Gdye group, with 83% Easy LFN rate. The Gdye group presented with low nerve injury degree and better PMV and PFP scores, with high sensitivity and accuracy. CONCLUSIONS: This experimental method of facial nerve fluorescence was effective for intraoperative nerve visualization, identification and preservation. The technique may be used in future facial nerve studies, translated to humans, contributing to the optimization of parotid surgery in the near future.
- ItemSomente MetadadadosO uso do b-FGF na regeneração do nervo facial em ratos(Universidade Federal de São Paulo (UNIFESP), 2007) Toledo, Ronaldo Nunes [UNIFESP]; Fukuda, Yotaka [UNIFESP]Introdução: Fatores que melhorem a capacidade de regeneração dos tecidos neurais tem sido constantemente investigados. Um dos possíveis auxiliadores desse processo e o fator básico de crescimento de fibroblasto (b-FGF). Objetivo: Analisar, através de parâmetros comportamentais e histométricos, a influência do uso tópico do b-FGF na regeneração do nervo facial de ratos. Metodo: Vinte e oito ratos Wistar, machos, adultos, foram submetidos a secção completa do tronco do nervo facial e em seguida, realizada anastomose termino-terminal com ponto epineural. Realizou-se implante subcutâneo de uma minibomba osmótica contendo um cateter que irrigava a anastomose neural durante 14 dias. Metade dos animais recebeu uma solução contendo 25μg/ml de b-FGF, 250UI/ml de heparina sódica e 1000 μg/ml de albumina humana diluídos em ringer lactato e o restante, uma solução controle com as mesmas concentrações, exceto pela ausência do b-FGF. Para avaliar a regeneração facial, contou-se o número de fibras mielínicas no 14° e 28° dia de pós-operatório e quantificaram-se os movimentos faciais em dias alternados até o 28° dia. Microscopia eletrônica foi realizada em 6 nervos no 14° dia para auxiliar na identificação das estruturas neurais. Resultados: Na avaliação comportamental, a partir do 8° até 16° dia, o grupo de animais que recebeu 0 b-FGF teve melhores resultados funcionais; porém, após esse período, apesar de resultados numericamente superiores, não houve diferenças estatísticas entre os dois grupos. Na avaliação histológica, 0 número de fibras mielínicas foi maior no 28° dia no grupo que recebeu o b-FGF e semelhante entre os grupos no 14° dia. Conclusão: A aplicação tópica do b-FGF no nervo facial de ratos adultos promoveu regeneração neural com maior número de fibras mielínicas e precocidade em sua recuperação funcional..