Navegando por Palavras-chave "Estrogen receptors"
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- ItemSomente MetadadadosDifferential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats(Elsevier B.V., 2014-01-25) Lucas, Thais Fabiana Gameiro [UNIFESP]; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Porto, Catarina Segreti [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to investigate the role of each estrogen receptors on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats. Activation of ESR1 by 17 beta-estradiol (E2) and ESR1-selective agonist PPT increased CCND1 expression, and this effect was dependent on NF-kB activation. E2 and the ESR2-selective agonist DPN, but not PPT, increased, in a PI3K and CREB-dependent manner, the expression of CDKN1B and the transcription factors GATA-1 and DMRT1. Analyzing the expression of ESR1 and ESR2 in different stages of development of Sefton cells, we observed that the ESR1/ESR2 ratio decreased with age, and this ratio seems to be important to determine the end of cell proliferation and the start of cell differentiation. in Sertoli cells from 15-day-old rats, the ESR1/ESR2 ratio favors the effect of ESR1 and the activation of this receptor increased [Methyl-31-I]thymidine incorporation. We propose that in Sertoli cells from 15-day-old rats E2 modulates Sertoli cell proliferation through ESR1/NF-kappa B-mediated increase of CCND1, and cell cycle exit and differentiation through ESR2/CREB-mediated increase of CDKN1B, GATA-1 and DMRT1. the present study reinforces the important role of estrogen for normal testis development. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
- ItemSomente MetadadadosEstrogen and Its Receptors in Efferent Ductules and Epididymis(Amer Soc Andrology, Inc, 2011-11-01) Hess, Rex A.; Fernandes, Sheilla Alessandra Ferreira [UNIFESP]; Gomes, Gisele Renata de Oliveira [UNIFESP]; Oliveira, Cleida A.; Lazari, Maria de Fatima Magalhaes [UNIFESP]; Porto, Catarina Segreti [UNIFESP]; Univ Illinois; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Minas Gerais (UFMG)Estrogens play key roles in the development and maintenance of male reproductive function and fertility. in this review, we briefly describe the localization and function of estrogen receptors ESR1 and ESR2 (also known as ER alpha and ER beta, respectively) and the expression of G protein-coupled estrogen receptor-1 (GPER, formerly known as GPR30) in efferent ductules and epididymis. the efferent ductules present the highest levels of ESR1 and ESR2 in the male reproductive system, and represent a major target of estrogen action. in efferent ductules, ESR1 has a crucial role in the regulation of fluid reabsorption, and in the epididymis the receptor helps to maintain fluid osmolality and pH. ESR1 expression in the epididymal epithelium shows considerable variation among species, but differences in laboratory techniques may also contribute to this variation. Here we report that Esr1 mRNA and protein are higher in corpus than in other regions of the rat epididymis. the mRNA level for Gper was also higher in corpus. Although ESR1 is expressed constitutively in efferent ductules and down-regulated by estrogen, in the epididymis, both testosterone (T) and estradiol (E2) may regulate its expression. T and E2 are, respectively, higher and lower in the corpus than in the initial segment/caput and cauda regions. It is important to determine the expression of GPER, ESR1, androgen receptor, and their respective cofactors in specific cell types of this tissue, as well as the intracellular signaling pathways involved in efferent ductules and epididymis. These studies will help to explain the consequences of exposures to environmental endocrine disruptors and provide potential targets for the development of a male contraceptive.
- ItemSomente MetadadadosEstrogen receptor ESR1 regulates the phospholipase C-inositol phosphate signaling in the hippocampus from rats in proestrous and estrous phases(Elsevier B.V., 2013-01-01) Maruyama, Nadia O.; Lucas, Thais F. G. [UNIFESP]; Porto, Catarina S. [UNIFESP]; Abdalla, Fernando M. F.; Inst Butantan; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to investigate the involvement of estrogen receptors in the activation of phospholipase C (PLC)-phosphoinositide hydrolysis in the hippocampus from rats in estrous and proestrous phases. 17 beta-Estradiol (E2) and ESR1 -selective agonist PPT, but not ESR2-selective agonist DPN, induced a rapid increase on total [H-3]-inositol phosphate accumulation in the hippocampus from both rats. These effects are mediated by PLC activation, since the inhibition of this protein decreased the total [H-3]-inositol phosphate accumulation. the pretreatment with ESR1 and ESR2 antagonist ICI 182,780, but not with GPER antagonist G-15, blocked the total [H-3]-inositol phosphate accumulation induced by E2 and PPT, confirming that ESR1 is upstream component regulating this rapid effect. SRC family of protein tyrosine kinases inhibitor PP2 blocked the total [H-3]-inositol phosphate accumulation induced by E2 and PPT in hippocampus, suggesting that ESR1 undergoes translocation from the nuclei to the plasma membrane region via SRC to activate rapid signaling pathways. Furthermore, the magnitude of the response to E2 and PPT was higher in hippocampus from rats in proestrous than in estrous. On the other hand, the expression of the ESR1 is higher in hippocampus from rats in estrous than in proestrous, indicating that the regulation of this receptor by estrous cycle does not play a role in the magnitude of the response to E2 and PPT in hippocampus. in conclusion, our results indicate that E2 activates SRC-mediated translocation of ESR1 to the plasma membrane, which results in the activation of PLC-inositol phosphate signaling pathway in rat hippocampus. Thus, these rapid estrogen actions in hippocampus might be a key step mediating cellular events important for learning and memory. (C) 2012 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosEstrogen receptors mediate rapid activation of phospholipase C pathway in the rat endometrium(Elsevier B.V., 2011-12-20) Konigame, Vivian Cristina; Siu, Erica Rosanna [UNIFESP]; Royer, Carine [UNIFESP]; Lucas, Thais Fabiana Gameiro [UNIFESP]; Porto, Catarina Segreti [UNIFESP]; Abdalla, Fernando Mauricio Francis; Inst Butantan; Universidade Federal de São Paulo (UNIFESP)The aim of the present study was to investigate the activation of rapid signaling events by 17 beta-estradiol in the rat uterus. 17 beta-Estradiol induced a rapid increase of total [(3)H]-inositol phosphate accumulation in the whole uterus and endometrium, but not in the myometrium. the effect of 17 beta-estradiol in the endometrium was blocked by phospholipase C (PLC) inhibitor (1173122), estrogen receptors antagonist (ICI 182,780), exportin CRM1 inhibitor (leptomycin B) and selective inhibitor of the SRC family of protein tyrosine kinases (PP2). Furthermore, a selective agonist of ESR1 (PPT) and a selective agonist of GPER (G-1) also induced a rapid increase of total ((3)H]-inositol phosphate accumulation in the endometrium. the G-1 effects were blocked by GPER antagonist (G-15). 17 beta-Estradiol and G-1 promoted an additive effect on total [(3)H]-inositol phosphate accumulation. in conclusion, the present results indicate that a rapid activation of the PLC-mediated phosphoinositide hydrolysis occurred in the rat endometrium after 17 beta-estradiol stimulation, and this effect was mediated by ESR1 that underwent nuclear export after hormone stimulation, and that GPER activation may play an additive role for this response. These rapid actions might be one of the key steps that mediate the estrogen-dependent activation of cellular events in the endometrium. (C) 2011 Elsevier Inc. All rights reserved.