Navegando por Palavras-chave "Esofagite péptica"

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    Avaliação do efeito quimiopreventivo de Brassica oleracea (variedade acephala) e do omeprazol em um modelo experimental de indução do refluxo duodenogastroesofágico
    (Universidade Federal de São Paulo (UNIFESP), 2009-03-25) Wisnieski, Fernanda [UNIFESP]; Freire-Maia, Dertia Villalba [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Aim: To evaluate, in an experimental model, the applicability of erythrocyte micronucleus assay in diagnosis of esophageal damage caused by gastroesophageal reflux disease (GERD) and to investigate the chemopreventive effect of Brassica oleracea extract and Omeprazole pump inhibitor drug. Methods: Brassica oleracea dose of 500 mg/Kg was determined using in vitro and in vivo toxicity tests. Male Wistar rats underwent esophagojejunostomy model to produce duodeno-gastroesophageal reflux. Eight weeks after surgical procedure, animals were treated with 500 mg/Kg of Brassica oleracea extract or 30 mg/Kg of Omeprazole for four weeks. The animals were weighed once a week. After the last treatment, the esophagus of each animal was removed for histological analysis, as well as, blood and bone marrow samples were obtained for erythrocyte micronucleus test. Results: Body weights of Reflux group were significantly lower than Control group (p < 0.0001). This difference was not observed among treatments. All non-treated animals of Reflux group developed severe esophagitis and one animal developed squamous cell carcinoma type twelve weeks after surgery. Omeprazole-treated animals developed lower incidence of spongiosis (p = 0.028) and lower grade of esophagitis (p = 0.018) compared to non-treated animals. Whereas the animals treated with Brassica oleracea extract showed the same results, no significant difference was observed. Micronucleated erythrocytes and micronucleus frequencies did not differ signicantly among groups and treatments and were not associated with histological alterations. Conclusions: The esophagojejunostomy model allowed the study of GERD progression ultil severe esophagitis. The Omeprazole treatment reduced the inflammatory process in esophageal mucosa and this effect was only indicative in Brassica oleracea treatment. The cell types evaluated by micronucleus assay did not reproduce the esophageal characteristics determined histologically. Considering the importance of this test in investigation of genotoxicity of various substances in vivo, the evaluation of esophageal cells exposed directly to reflux could show the real alterations caused by GERD, as well as the possible effects of Brassica oleracea extract and Omeprazole treatments.
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    Esofagite de refluxo em crianças: estudo histológico e morfométrico
    (Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED, 2002-04-01) Mader, Ana Maria Amaral Antonio [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Kawakami, Elisabete [UNIFESP]; Patricio, Francy Reis da Silva [UNIFESP]; Fundação ABC Faculdade de Medicina Departamento de Patologia; Universidade Federal de São Paulo (UNIFESP)
    Background - Gastroesophageal reflux disease is a frequent cause of morbidity in childhood, including esophagitis and recurrent respiratory symptoms; however histological and morphometric studies in esophageal biopsies of children are scarce. Aim - To study histological and morphometric findings in children with reflux esophagitis. Patients and Methods - We studied 26 esophageal biopsies of children (mean age: 4.1 years ± 3.4) with reflux esophagitis, which prevailed in boys (84.6%); post-prandial vomiting (76.9%) and repetitive bronchopneumonia (38.5%) were the most frequent symptoms. The diagnosis was made by X-ray in 18, by pH evaluation in 5 and by scintilography in 3 patients. The control group was formed by seven children without reflux symptoms who died from meningococcemia or congenital heart malformation (mean age: 2.5 years ± 2.3). Histological variables were studied by hematoxylin-eosin and periodic acid of Schiff stain, inflammatory infiltrate, epithelial and basal layer thickness, papillary length and its ratio with the thickness of the epithelium. Morphometry was performed at a digital system connected to pro-image software. Student's t test, Mann-Whitney test, Fisher and Pearson's correlation methods were used for the statistical analysis. Results - Epithelial and basal zone thickness, papillary length and its rate with thickness of epithelium, among the reflux group patients were higher than the control group. Eosinophils, neutrophils and balloon cells were not observed in the control group. Intraepithelial capillaries occurred in 11 cases in the reflux group (mean diameter: 59 mum). Conclusion - Epithelial and basal zone thickness, papillary length and its ratio with thickness of epithelium, resulted greater in the gastroesophageal reflux group than in the control group. There was a direct correlation between thickness of epithelium, basal zone thickness and papillary length, showing increased epithelial cell turnover. Eosinophils, neutrophils and balloon cells were observed only in patients with gastroesophageal reflux, thus serving as specific markers of this disease.