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- ItemAcesso aberto (Open Access)Efeito da suplementação de probióticos sobre função de monócitos e endotoxemia após exercício extenuante(Universidade Federal de São Paulo (UNIFESP), 2021) Silva, Edgar Tavares da [UNIFESP]; Santos, Ronaldo Vagner Thomatieli dos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Different physiological and biochemical responses are generated before, during and after performing strenuous physical exercises with high ability to negatively impact the immune and gastrointestinal system, promoting transiently immunodepression. Strategies are adopted to minimize the deleterious effects caused by strenuous physical exercise on the immune system, including macro and micronutrient supplementation. Emerge the interest in evaluating the effects of probiotic supplementation on immune function and maintenance of gastrointestinal and immunological homeostasis in athletes. Therefore, the present study aimed to evaluate the monocyte's function, endotoxemia and intestinal microbiota of 27 individuals who performed an official Marathon race (42,195m). The athletes were supplemented with probiotics for four weeks with Lactobacillus acidophilus and Bifidobacterium animalis subsp lactis 1 x 1010 Colony Forming Units (CFU) or placebo. Before the supplementation period (Basal), after the supplementation period/referring to the 24 hours before the race (Pre-race), one hour after (Post-race) and five days after the marathon (Recovery) blood and faeces were collected to determine the plasma concentration of cytokines, monocyte function, microbiota analysis and endotoxemia. The probiotic group significantly increased the phagocytosis rate after thirty days of probiotic supplementation. The production of hydrogen peroxide and cytokines by monocytes did not differ between the groups. After the marathon race, plasma IL-10 increase and five days after, plasma IL-15 increase and plasma IL-8 decrease in both groups. The intestinal microbiota does not differ between groups. The symptoms and severity of URTI were not different between groups. We concluded that daily supplementation with probiotics for 4 weeks in runners increased phagocytosis, but were not able to modulate the intestinal microbiota and part of the cellular functionality studied after marathon running.
- ItemAcesso aberto (Open Access)Efeitos da endotoxemia sobre o sistema Renina-Angiotensina renal de ratos Wistar(Universidade Federal de São Paulo (UNIFESP), 2013-09-25) Rosa, Rodolfo Mattar [UNIFESP]; Casarini, Dulce Elena [UNIFESP]A sepse é um grande problema de saúde pública mundial, provocando a morte de cerca de 215 mil pessoas por ano com custos que chegam aos 17 bilhões de dólares. Essa doença é definida como uma resposta inflamatória sistêmica e descontrolada frente a uma infecção, sendo que um dos principais órgãos acometidos por ela é o rim, em especial quando ocorre infecção por bactérias Gram-negativas. Tendo em vista as alterações renais e cardiovasculares provocadas pela sepse, o objetivo do presente estudo foi verificar a modulação de alguns componentes do SRA em rim de animais tratados com lipopolissacarídeo de E. coli (LPS), um modelo de IRA decorrente da sepse. Inicialmente, ratos Wistar receberam injeção de 4 mg/kg de LPS e foram eutanasiados 3 dias após o tratamento. Os animais endotoxêmicos mostraram redução do peso corporal e da pressão arterial. Os níveis plasmáticos de citocinas pró-inflamatórias não se encontrou alterado três dias após o tratamento. Contudo os valores de NO plasmático e iNOS renal também mostram-se reduzidos nos animais tratados com LPS. Isso indica que, em nosso modelo, 3 dias após a injeção de toxina, o NO não é mais o principal responsável pela manutenção da hipotensão e outras vias de vasodilatação podem estar mais ativadas. O ritmo de filtração glomerular não se alterou, o que foi evidenciado pela manutenção dos valores da depuração de creatinina entre os grupos. Contudo, a vasoconstrição renal parece estar instalada pelo tratamento com LPS, pois foram observados altos níveis de Ang II no rim desses animais. Além disso, esse peptídeo também pode estar associado à progressão da resposta inflamatória podendo desencadear dano tecidual. A ECA, principal produtora de Ang II, apresentou expressão e atividade diminuídas, indicando que vias alternativas da produção desse hormônio podem estar ativadas. Além disso, a renina também apresentou redução de atividade no rim endotoxêmico, indicando uma tentativa de controle de atividade do SRA. Dessa forma, o tratamento prolongado com LPS pareceu ativar vias compensatórias visando uma tentativa de volta à homeostase e de minimização dos danos provocados pela endotoxemia.
- ItemSomente MetadadadosO Papel Do Exercício Físico Agudo E Crônico Na Lesão Renal Aguda Induzida Pela Sepse(Universidade Federal de São Paulo (UNIFESP), 2017-08-31) Hungaro, Talita Guerreiro Rodrigues [UNIFESP]; Araujo, Ronaldo De Carvalho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: Hormone is a phenomenon where low levels of stress stimulate or regulate the molecular pathways positively and improve the organic capacity to withstand greater stress. This preconditioning can be achieved through different stimuli, including physical exercise. Previous work has demonstrated the preconditioning effect of physical exercise on injury or disease in different organs and systems, attenuating inflammatory responses and lung injury in endotoxemia, promoting adaptations, reducing injury and mortality during polymicrobial sepsis and preventing muscle damage in an experimental model of sepsis. Our study demonstrated that physical exercise is able to protect the kidney from nephrotoxicity caused by cisplatin by reducing levels of urea and creatinine, IL-1beta, IL-6, IL-10 and TNF-alpha in serum. Although several studies address chronic physical exercise, little is known about the role of acute exercise in disease development. Objectives: To verify the role of acute and chronic physical exercise in the treadmill in the endotoxemic response in renal injury. Method: C57BL / 6 male mice of 12 weeks were divided into 6 groups: 1- Sedentary control without LPS; 2- Sedentary with LPS; 3- Acute training without LPS; 4- Acute training with LPS; 5 - Chronic training without LPS and 6 - Chronic training with LPS. In the acute training the animals perform 1 session of physical exercise of 60 minutes. In chronic training the animals exercise 5 times a week, from Monday to Friday, 60 minutes each session, for 4 weeks. The training velocity was defined as 60% of the average of the maximum velocities reached, classifying the training as moderate. LPS induction was performed 24 hours after the end of the last exercise session. The responses of inflammatory or anti-inflammatory mediators were evaluated by elisas. Renal repercussion was measured by urea concentration, as well as by histological sections. Results: The dose of LPS applied intraperitoneally was 5mg / kg, with the serum extracted concentration tests were performed for the 6 experimental groups. There was a significant increase in urea concentration in all groups that received LPS when compared to those who did not, but there was no difference between the urea concentration in the groups trained with LPS and sedentary with LPS. Protein expression of IL-1beta showed no difference between groups, TNF-alpha presented expression only in the groups with LPS but without statistical difference. For IL-10, there was a difference between the sedentary + LPS groups and chronic LPS training, and the cytokine expression decreased in the trained group, contrary to the initial hypothesis. Partial conclusions: From the results obtained so far, it can be concluded that physical exercise was not able to prevent the inflammatory response caused by LPS infection.