Navegando por Palavras-chave "Embryology"
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- ItemSomente MetadadadosHistological characteristics of the gonads of pig fetuses and their relationship with fetal anatomical measurements(Elsevier Sci Ltd, 2018) Pontelo, Thais Preisser; Miranda, Jose Rafael; Felix, Matheus Augusto Rodrigues; Pereira, Barbara Azevedo; Silva, William Eduardo da; Avelar, Gleide Fernandes; Mariano, Flavia Cristina Martins Queiroz [UNIFESP]; Guimaraes, Gregorio Correa; Zangeronimo, Marcio GilbertoThe objective was to evaluate the histomorphometric characteristics of the testis and ovaries of pig fetuses at different gestational ages, as well as their correlation with some fetus measurements. Forty-four fetuses were separated for gender (male and female) and gestational age (50, 80 and 106 days of gestation). After slaughter, fetuses had their body length, head and thoracic perimeters measured and their gonads submitted to histomorphometric analyses. The gonadal characteristics at different gestational ages were statistically compared, correlations with the fetal measurements were performed and equations to predict the gonadal characteristics from the fetal measurements were obtained. The testis weight logarithmically increased along pregnancy, whereas ovary weight increased in a linear manner. The cordonal length and number of Sertoli cells were positively correlated with the fetal measurements, being higher at 106 days gestation, while the nuclear volume of these cells were negatively correlated. The total number of follicles was higher at day 80 and 106 of pregnancy. The number of oogonia decreased along the pregnancy, however, their nucleus size was increased. The number of follicles and volume of oogonia were positively correlated with the fetal measurements, while the number of oogonia was negatively correlated. Equations were obtained for the prediction of gonadal characteristics of fetuses. We concluded that in pigs testis cell proliferation, ovary development and histological organization was more pronounced during the final third of pregnancy. Fetal weight and size were strongly related to gonadal development, and can be used to estimate the histological characteristics of gonads.
- ItemSomente MetadadadosInfluência Do Meio De Cultivo Condicionado De Células-Tronco Mesenquimais No Desenvolvimento Embrionário Murino Até O Estágio De Blastocisto(Universidade Federal de São Paulo (UNIFESP), 2018-05-22) Silveira, Larissa Berioni Rodrigues Da [UNIFESP]; Turco, Edson Guimaraes Lo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: Evaluate The Influence Of The Conditioned Culture Medium From Endometrial Mesenchymal Stem Cells In The Embryonic Cell Culture Method: 544 Murine Embryos Were Divided In 3 Groups: Control (Regular Embryonic Culture Medium); Supplemented (Embryonic Culture Medium Supplemented With Conditioned Imdm Medium After Endometrial Mesenchymal Stem Cell Culture); And Negative Control (Regular Embryonic Culture Medium Supplemented With Imdm Medium). Embryonic Development Was Evaluated In Each Group And Culture Media Were Collected For Metabolomic Analysis By Mass Spectrometry. The Data Were Normalized By Z-Score And Submitted To The Pca And Pls-Da Tests Using The Software Metaboanalyst 3.0, To Identify The Molecular Markers. Results: In The Comparison Between The Three Groups, The Control Group Presented A Higher Blastocyst Rate On The Sixth Day Of Embryo Culture In Relation To The Supplemented And Negative Control Groups (0,3 ± 0,160; 0,01±0,01; 0,00±0,00, Respectively) , While The Rate Of Degenerate Was High
- ItemAcesso aberto (Open Access)Uso de material didático sobre embriologia do sistema nervoso: avaliação dos estudantes(Associação Brasileira de Educação Médica, 2012-03-01) Oliveira, Mariana Sampaio De; Kerbauy, Mariana Nassif [UNIFESP]; Ferreira, Camila Nassif Martins; Schiavão, Lucas José Vaz; Andrade, Rodrigo Franzoso Almeida De; Spadella, Maria Angélica; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de São Paulo (UNIFESP); Faculdade de Medicina de MaríliaHuman embryology is an extremely important part of training health professionals, but its study is often considered boring, decorative and without practical significance for many students, making it ineffective. This study aimed to produce interactive teaching material with embryonic models representing the embryonic development of the nervous system. To qualitatively evaluate the real effectiveness and ability to facilitate the teaching-learning process in the context of problem-based learning, the material was applied to first-year medicine and nursing students. The students felt that the material contributed to a better understanding of the topic and enabled visualization of embryonic events, as well as arousing interest in studying the subject. The material served to establish understanding and inspire the students to integrate the knowledge with other disciplines. Thus, in qualitative terms, analysis of the student questionnaires indicated that the material produced was effective for learning and worked as a resource in self-directed learning, promoting the diversification of search sources and being complementary to textbooks and other theoretical materials.