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    Avaliação do potencial hipoglicêmico dos extratos brutos e neolignanas isoladas de Nectandra leucantha (Lauraceae) em células β pancreáticas
    (Universidade Federal de São Paulo, 2017-03-17) Ramirez, Pamela Noemy Laura [UNIFESP]; Lago, João Henrique Ghilardi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Diabetes mellitus (DM) is characterized by chronic hyperglycemia and disorders in the metabolism of carbohydrates, proteins and lipids. This disease affects a large number of people in the world, and in Brazil the prevalence of DM in adults is concentrated in the southeastern region of the country despite the use of several drugs in the treatment of DM. It is necessary to search for new therapeutic alternatives. In this sense, the search for new molecules with antidiabetic activity has been directed to the natural products. Aiming for the recognition of bioactive metabolites, the hexane and methanol extracts of N. leucantha were obtained. The hexane extract was subjected to chromatographic fractionation, where it was possible to isolate two neolignans: 1,2-dimethoxy-6- [2'-methoxy-4 '- (8'-propenyl) phenoxy] -4- (8-propenyl ) benzene (C1) and dehydrodieugenol B (C2), the structures of these compounds being defined by analysis of the NMR spectra. The antiradicalar power of hexane, methanol and two neolignan extracts was evaluated, where the methanolic extract (EC50 = 5.76 ± 0.20 μg / mL) and neolignan C2 (EC50 = 15.30 ± 0.31 μg / mL) presented higher antiradicalar power. An analytical method was developed capable of quantifying the content of the neolignans present in the hexane extract of the N. leucantha branches, in order to know the amount of these neolignans. In the biological tests for the hexanic and methanol extracts and the neolignans isolated against the INS-1E cell line, the cell viability was first evaluated and the concentrations were chosen: 40 μg / mL for the methanol extract, 37.5 μg / mL for the hexane extract, 25μM for neolignan C1 and 40μM for neolignan C2. It was also evaluated the production of reactive oxygen species (EROs) of the hexane and methanol extracts and the action of extracts and neolignans isolated under conditions of oxidative stress induced by low glucose concentration were evaluated.