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    Desenvolvimento de uma membrana de quitosana com propriedades de barreira celular e capacidade de liberação de droga
    (Universidade Federal de São Paulo, 2014-03-06) Gondim, Luciano de Rezende [UNIFESP]; Bizeto, Marcos Augusto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Chitosan is a water-soluble semisynthetic biopolymer with recognized potential application in the medical field because of properties such as bactericidal activity, biocompatibility, drug releasing, among others. Chitosan Membranes were prepared by the casting method. In order to improve mechanical and drug delivery properties of these membranes, different amounts (1 to 90 wt.%) of mesoporous silica MCM-41 were added to the chitosan solution during the preparation process. The MCM-41 presents an extensive network of pores with regular sizes and structural ordering that provide high surface area and drug loading capacity for later release. Membranes as well as their intermediate components were chemically and structurally characterized. The membranes containing less than 20 wt% of MCM-41 have adequate flexibility to manipulation, do not show pore with cell dimensions and surface segregation of the polymer and inorganic phases. A membrane containing 20 wt.% of MCM- 41 and another one composed of pure chitosan were subjected to the in vitro and in vivo assays, comparatively. The drug release property of membranes is influenced by the MCM-41 presence. The membrane where ibuprofen was previously immobilized in MCM-41 showed a release profile with prolonged release over time when compared to pure chitosan membrane. The biocompatibility was evaluated in vivo by surgical insertion between the abdominal wall muscles of Wistar-SEM (n = 8) and subsequent histological analysis of tissue after 7 and 28 days of implantation. The potential inhibition of cell transmigration was confirmed by histology tissue where the membrane has been deployed. The inflammatory response membranes with or without silica was similar to the one observed for the collagen membranes and commercial polypropylene used as controls. After 28 days the chitosan membrane showed integration with the surrounding tissue and no chronic inflammation, thus demonstrating adequate biocompatibility. The hybrid membranes of chitosan prepared with MCM-41 showed cell barrier property, biocompatibility and release capacity of ibuprofen, showing a great potential for medical applications. Microbiological testing of the membranes showed the preservation of antibacterial property of chitosan..