Navegando por Palavras-chave "Cumulus cell"
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- ItemSomente MetadadadosThe follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells(Springer, 2012-11-01) Montani, Daniela Antunes [UNIFESP]; Cordeiro, Fernanda Bertuccez [UNIFESP]; Regiani, Thais [UNIFESP]; Victorino, Amanda Begati [UNIFESP]; Pilau, Eduardo Jorge; Gozzo, Fabio Cesar; Ferreira, Christina Ramires; Fraietta, Renato [UNIFESP]; Lo Turco, Edson Guimaraes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy.Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built.S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the non-pregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation.We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.
- ItemAcesso aberto (Open Access)O papel da autofagia em oócitos bovinos expostos à hiperglicemia durante a maturação in vitro(Universidade Federal de São Paulo, 2022-01-27) Soares, Thays da Silva [UNIFESP]; Lopes, Fabíola Freitas de Paula [UNIFESP]; http://lattes.cnpq.br/0954914266701996; http://lattes.cnpq.br/8795253441230568Diabetes Mellitus (DM) is a disease that affects the population worldwide, being characterized by hyperglycemia, involving dysregulations of insulin production and/or action. Both DM and hyperglycemia can be harmful to the body. There is a proportional relationship between hyperglycemia and autophagic markers in oocytes from STZ-treated mice, but no studies related to the function of this relationship and its mechanisms were found. Bovine oocytes were used as an experimental model for humans due to similarities in gametogenesis and preimplantation embryonic development between these species. Therefore, this project aimed to determine the role of autophagy in bovine oocytes submitted to hyperglycemia condition during in vitro maturation, evaluating cumulus cell expansion and mitochondrial activity. Therefore, cumulus-oocyte complexes (COCs) collected from slaughterhouse animals were submitted to in vitro maturation (IVM) with 5.5 mM (control glucose group) or 20 mM glucose (hyperglycemic group) in the presence of 0 or 10 mM of autophagy inhibitor (3-methyladenine: 3-MA). For the first experiment, cumulus cell expansion was evaluated before (0h) and after IVM (21-24h). Image acquisition and analysis of the size of the OCCs were performed using the ImageJ program, version 1.53n. For the second experiment, the denuded oocytes were stained with Mitotracker Red to determine mitochondrial activity. The fluorescence intensity was quantified with the Image J 153n program. Inhibition of autophagy in COCs matured in hyperglycemic medium reduced the area of expansion of cumulus cells (P= 0.0051). Oocyte mitochondrial activity was increased by glucose concentration (P = 0.0330) and autophagy inhibitor (P = 0.0437). Inhibition of autophagy in COCs matured in hyperglycemic medium increased mitochondrial activity. In conclusion, autophagy may contribute to the protection of cumulus and oocyte cells against the deleterious effects of hyperglycemia
- ItemSomente MetadadadosProtein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success(Springer/Plenum Publishers, 2016) Braga, Daniela Paes Almeida Ferreira [UNIFESP]; Setti, Amanda Souza; Lo Turco, Edson Guimaraes [UNIFESP]; Cordeiro, Fernanda Bertuccez [UNIFESP]; Cabral, Elaine Cristina; Cortezzi, Sylvia Sanches; Ono, Erika; Savio Figueira, Rita Cassia; Eberlin, Marcos Nogueira; Borges Júnior, Edson [UNIFESP]The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.