Navegando por Palavras-chave "Consenso de FAN-HEp-2"

Agora exibindo 1 - 1 de 1
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
    (Elsevier B.V., 2014-01-01) Francescantonio, Paulo Luiz Carvalho; Cruvinel, Wilson de Melo [UNIFESP]; Dellavance, Alessandra [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Taliberti, Ben Hur; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Bueno, Cleonice; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Bonfá, Eloisa Silva Dutra de Oliveira; Brito, Fabiano de Almeida; Araújo, Flávia Ikeda e; Rego, Jozelia; Pereira, Kaline Medeiros Costa; Anjos, Lisiane Maria Enriconi dos; Bissoli, Maria de Fatima; Santiago, Mittermayer Barreto; Maluf, Natalya Zaidan; Alvarenga, Rossana Rassi; Neves, Suzane Pretti Figueiredo; Valim, Valeria; Santos, Wilton Silva dos [UNIFESP]; PUC Goias; Fleury Med & Saude; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Uberlândia (UFU); Clin Doencas Reumat Porto Alegre; Amaral Costa Med Diagnost; Universidade de São Paulo (USP); Hosp Israelita Albert Einstein; Inst Hermes Pardini; Universidade Federal de Minas Gerais (UFMG); Univ Catolica Brasilia; Universidade Federal de Goiás (UFG); Univ Sul Santa Catarina UNISUL; Univ Vale do Itajai UNIVALE; Univ Fed Espirito Santo; EBMSP; Grp DASA; Escola Super Ciencias Saude Dist Fed; Lab Sabin
    Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.