Navegando por Palavras-chave "Citotoxicity"

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    Avaliação da citotoxicidade e genotoxicidade da coexposição ao Triclosan e ao Ftalato DEHP em células HepG2
    (Universidade Federal de São Paulo (UNIFESP), 2021) Lima, Lindiane Eloisa De [UNIFESP]; Barcelos, Gustavo Rafael Mazzaron [UNIFESP]; Universidade Federal de São Paulo
    Triclosan (TCS) is widely used as a preservative and bactericide in personal care products, household items, sports equipment, and toys. Di-2-ethylhexyl phthalate (DEHP) is the main derivative of phtalic acid, can give malleability to plastic, which is why it is widely used in medical devices, toys, building materials, etc. Considered endocrine disrupters and environmental contaminants, studies have shown several adverse effects, however the majority have an emphasis on ecotoxicology using aquatic organisms, thus, there is little data about the evaluation of the possible adverse effects of these contaminants in mammals. This study aims to assess the impact of exposure to TCS, DEHP, as well as their associations, on cell viability and genomic stability, in HepG2 cells. The MTT and Red Neutral (VN) assays were used to assess cytotoxicity using different concentrations of TCS and DEHP (0.3 - 50 µM) and the cells were exposed for 24 hours. From the results of these tests, three concentrations of each toxicant were chosen (0.10; 1.0 and 10 µM), for subsequent monitoring of cell viability and DNA damage, by the comet assay. The results show that the lysosomal pathway was not affected by coexposure to toxicants; however, mitochondrial activity was compromised at all concentrations tested, especially in combinations of the lowest DEHP concentration (0.10 µM) with TCS concentrations 0.10 µM (44%), 1.0 µM (17%) and 10 µM (21%). Genotoxicity by the comet assay showed greater DNA damage at concentrations of 0.10 µM TCS (16.4%) and 1.0 µM DEHP (19%), than in higher concentrations. The combinations showed significant damage when compared to the negative controls, but it is not significant when compared to the corresponding individual concentrations of TCS and DEHP. The results observed by cytotoxicity and genotoxicity tests suggest possible changes in the cell redox state and consequent induction of DNA damage.