Navegando por Palavras-chave "Células sangüíneas"
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- ItemAcesso aberto (Open Access)Aspectos morfológicos e citoquímicos dos glóbulos sangüíneos de Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)(Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo, 1999-01-01) Moura, Weber Leal De [UNIFESP]; Matushima, Eliana Reiko; Oliveira, Luiz Waldemar [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Samples of peripheral blood were collected from 10 healthy Caiman crocodilus yacare and stained with Leishman, for morphological analysis, or submitted to the following cytochemical methods: PAS, Sudan black B, o-toluidine and mercury bromophenol blue. Seven types of cells were identified: erythrocytes, thrombocytes, heterophils, eosinophils, basophils, lymphocytes and azurofilic monocytes. Erythrocytes were elliptical and positive for bromophenol blue. Some mitotic figures were noted. Spindle-shaped thrombocytes showed large nucleus with striking indentation. The cytoplasm was attenuated at one or both poles into knob-like projections containing glycogen. Heterophils revealed round eccentric nuclei and cytoplasm containing glycogen and many polymorphic granules. These granules showed partial positive reaction for Sudan and activity for myeloperoxidase. Eosinophils showed eccentrically located lenticular-shaped nuclei and numerous round granules stained positive with myeloperoxidase and Sudan. Basophils revealed a round central nuclei and cytoplasm with few basophilic round granules. Lymphocytes appeared as polygonal or irregular cells with thin cytoplasm forming bleb-like protrusions. Azurophilic monocytes were round with eccentrically located nuclei and basophilic cytoplasm containing numerous azurophilic granules.
- ItemAcesso aberto (Open Access)Cytochemical aspects of the peripheral blood cells of Oreochromis (Tilapia) niloticus. (Linnaeus, 1758) (Cichlidae, Teleostei): part II(Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo, 2001-01-01) Ueda, Ivete Kotomi [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Sasso, Wilson da Silva [UNIFESP]; Matushima, Eliana Reiko; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Morphologicaly, seven types of cells were identified in the blood of Oreochromis niloticus: erythrocytes, thrombocytes, neutrophils, eosinophils, basophils, lymphocytes and monocytes. Glycogen was present not only in the cytoplasm of neutrophils and thrombocytes but also in some lymphocytes and monocytes. The positive reaction for myeloperoxidase and Sudan black was observed in neutrophils and eosinophils. The bromphenol blue method was strongly positive for erythrocytes and eosinophils.