Navegando por Palavras-chave "Células HEp-2"

Agora exibindo 1 - 4 de 4
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Como interpretar e valorizar adequadamente o teste de anticorpos antinúcleo
    (Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2007-06-01) Dellavance, Alessandra [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fleury Medicina Diagnóstica Setor de Imunologia
    The tradicional fluorescent antinuclear antibody test (ANA) has required constant update efforts from personel involved in performing and interpreting its results. The methodological advances have brought up a considerable improvement in the test s sensitivity and, consequentely, a decrease in its specificity. This has resulted in an increasing number of positive tests in apparently healthy subjects. However, there are some peculiar features associated with the auto-antibodies in patients with autoimmune diseases that are not present in those observed in healthy subjects. The objective of the present review is to bring an approach on the most important points to be considered in the analysis and evalution of an ANA test that might help in the identification of patients with autoimmune disease. Title and immunofluorescence pattern are discussed as important parameters and they are important in the evaluation of ANA and in the reflex demand for further tests for specific autoantibodies. The basic concepts of the National Consensus on Standardization of ANA-HEp-2 Report are posted. Finally, we explore the possible meanings of a positive ANA test in a patient without objective evidence of autoimmune disease.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    III Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2: perspectiva histórica, controle de qualidade e associações clínicas
    (Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2009-06-01) Francescantonio, Paulo Luiz Carvalho; Andrade, Luiz Eduardo Coelho [UNIFESP]; Cruvinel, Wilson de Melo [UNIFESP]; Araújo, Flávia Ikeda e; Dellavance, Alessandra [UNIFESP]; Gabriel Júnior, Alexandre [UNIFESP]; Nuccitelli, Barbara; Taliberti, Ben Hur; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Santos, Cláudio Henrique Ramos dos; Bueno, Cleonice; Yano, Cristiane Martinez; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Cardoso, Elizângela; Bonfá, Eloisa Silva Dutra de Oliveira; Rassi, Gustavo Gabriel; Mundim, Hugo Mendonça; Bendet, Izidro; Rego, Jozelia; Vieira, Lisiane Maria Enriconi dos Anjos; Barbosa, Maria Ordália Ferro; Sugiyama, Mitiko; Santiago, Mittermayer Barreto; Slhessarenko, Natasha; Silva, Nilzio Antônio da; Jarach, Renata; Suda, Roberto; Levy, Roger Abramino; Sampaio, Silvia Oliveira; Neves, Suzane Pretti Figueiredo; Santos, Wilton Silva dos [UNIFESP]; Nóbrega, Yanna Karla de Medeiros; UCG; Universidade Federal de São Paulo (UNIFESP); Fleury Medicina e Saúde setor de Imunologia; UCG Departamento de Biomedicina Laboratório de Apoio Didático; Centro Imuno-Reumatológico de São Paulo AFIP-Medicina Laboratorial; Padrão Laboratório Clínico; Universidade Federal de Uberlândia; UFU Hospital das Clínicas serviço de reumatologia; Pontifícia Universidade do Rio Grande do Sul Faculdade de Medicina; Metanalysis Centro de Diagnósticos Médicos; Universidade Federal do Pará; Laboratório Amaral Costa; Centro de Educação Técnica do Estado do Pará; NewLife Comércio de Produtos Laboratoriais; Universidade de São Paulo (USP); Hospital Israelita Albert Einstein Departamento de Patologia Clínica; Instituto Hermes Pardini; Biometrix Diagnóstica; Laboratório Atalaia; Sérgio Franco Medicina Diagnóstica setor de Imunoensaios; UCG Faculdade de Medicina Hospital das Clínicas; Universidade do Sul de Santa Catarina; Universidade do Vale do Itajaí; Laboratório Médico Santa Luzia; Hospital e Maternidade e Jardim América Laboratórios Saúde; Hemagen Diagnósticos; Universidade Federal da Bahia; Fundação Bahiana para Desenvolvimento das Ciências; Hospital Santa Izabel serviço de reumatologia; Universidade Federal de Mato Grosso; FMUCG; Alka; Universidade do Estado do Rio de Janeiro; Hospital Pró-cardíaco Centro de Autoimunidades; Diagnósticos da América SA; Laboratório Diagnósticos da América SA setor de Imunologia; Universidade Federal de Minas Gerais Faculdade de Medicina; UFMG Hospital das Clínicas Serviço de Medicina Laboratorial; Hospital Universitário de Brasília Laboratório de Reumatologia; União Educacional do Planalto Central; Centro Universitário Euro-Americano; Imunotech Sistemas Diagnósticos Importação e Exportação
    OBJECTIVE: The Third Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) had as purpose the evaluation of difficulties in the accomplishment of the 2nd Consensus recommendations that took place in the year of 2002, the discussion of strategies for quality control of the assay and the discussion of an update of the clinical associations of the several immunofluorescent patterns. METHODS: Several ANA experts from university centers and private laboratories in different areas in Brazil joined the workshop in Goiânia on 2007 April 13 and 14 with the purpose of discussing and approving the recommendations for standardization, interpretation and use of the test by physicians. Commercial representatives of different ANA slide brands were also invited as listeners to the workshop. RESULTS AND CONCLUSION: The 3rd ANA Consensus emphasized the need for quality control in indirect immunofluorescent assays since there is a considerable heterogeneity of available microscopes and reagents. It also promoted adaptations in the previously approved terminology used to classify the different patterns and finally updated the clinical associations of the several patterns with the purpose of providing guidance for interpretation of the assay by clinical pathologists and assistant physicians.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
    (Elsevier B.V., 2014-01-01) Francescantonio, Paulo Luiz Carvalho; Cruvinel, Wilson de Melo [UNIFESP]; Dellavance, Alessandra [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Taliberti, Ben Hur; Von Mühlen, Carlos Alberto; Bichara, Carlos David Araújo; Bueno, Cleonice; Mangueira, Cristóvão Luis Pitangueiras; Carvalho, Darlene Gonçalves; Bonfá, Eloisa Silva Dutra de Oliveira; Brito, Fabiano de Almeida; Araújo, Flávia Ikeda e; Rego, Jozelia; Pereira, Kaline Medeiros Costa; Anjos, Lisiane Maria Enriconi dos; Bissoli, Maria de Fatima; Santiago, Mittermayer Barreto; Maluf, Natalya Zaidan; Alvarenga, Rossana Rassi; Neves, Suzane Pretti Figueiredo; Valim, Valeria; Santos, Wilton Silva dos [UNIFESP]; PUC Goias; Fleury Med & Saude; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Uberlândia (UFU); Clin Doencas Reumat Porto Alegre; Amaral Costa Med Diagnost; Universidade de São Paulo (USP); Hosp Israelita Albert Einstein; Inst Hermes Pardini; Universidade Federal de Minas Gerais (UFMG); Univ Catolica Brasilia; Universidade Federal de Goiás (UFG); Univ Sul Santa Catarina UNISUL; Univ Vale do Itajai UNIVALE; Univ Fed Espirito Santo; EBMSP; Grp DASA; Escola Super Ciencias Saude Dist Fed; Lab Sabin
    Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Reprodutibilidade de padrões morfológicos no teste de autoanticorpos contra antígenos celulares (FAN-HEp-2) em diferentes substratos
    (Universidade Federal de São Paulo (UNIFESP), 2020-11-18) Silva, Monica De Jesus [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Universidade Federal de São Paulo
    The indirect immunofluorescence assay on HEp-2 cells (HEp-2 IFA) is the gold standard method for anti-cellular (AC) antibody screening. Cell culture and fixation methods influence in the distribution and preservation of autoantigens, therefore affect in antigen preservation and distribution, as well as the AC patterns (1,2). Objective: We aimed to evaluate the non-reproducibility phenomenon of HEp-2 IFA results in different HEp-2 slide brands from patients with Systemic Autoimmune Disease (SAD), patients with Non-Autoimmune (NAD), and samples from healthy individuals. Methods: Were evaluated 868 serum samples from 275 patients with SAD, 293 patients with NAD, and 300 samples from healthy blood donors (HBD).Samples were processed at 1:80 dilution according to standard procedure on four HEp-2 slide brands. The tests were interpreted by three experienced independent blinded observers. The agreement among slides was determined using proportional weights according to the number of concordant slides in each sample, yielding a weighted score (ranging from 1 to 100) in specific groups of samples. The qualitative variables were analyzed by the Chi-square test and concordance was analyzed using the Kappa test. All data were analyzed using SPSS20.0 software at a significance level of p <0.05. Results: 402 samples were non-reagent in all slide brands and, therefore, were considered devoid of autoantibodies and eliminated from further analysis. The global reactivity agreement score obtained forth e remaining 466 samples (238 SAD, 119 NAD, and 109 HBD) was considered regular (74, 5). The nuclear compartment showed the highest agreement score (83,6), followed by the metaphase plate (78,9), cytoplasm (77,4) and nucleolus (72,4).Among the clinical groups, the agreement score was higher in SAD (78,0) than in NAD (70,6) and HBD (71,3). Samples with low reactivity (+/4) had higher discordance rates than high intensity samples (++++/4). In the SAD group the discordance for nuclear reactivity was 12.5% for ++++/4 samples, as opposed to 95.6% for +/4 samples, and the same was observed with NAD and HBD samples. The most robust nuclear patterns (higher concordance rate) were, Centromeric (score 78,4), Multiple Nuclear Dots (score73,6), Coarse Speckled (score71,3), and Homogeneous (score 67,9). Among the cytoplasmic patterns, the Reticular Cytoplasmic was more robust (68, 6). Conclusion: The phenomenon of non-reproducibility in the HEp-2 IFA among different slide brands occurs with highest frequency with samples with low intensity.