Navegando por Palavras-chave "Bone resorption"
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- ItemSomente MetadadadosB-1 lymphocytes differentiate into functional osteoclast-like cells(Elsevier B.V., 2012-03-01) Pugliese, Livia Souza [UNIFESP]; Gonçalves-Zillo, Thais Oliveira [UNIFESP]; Popi, Ana Flavia [UNIFESP]; Mariano, Mario [UNIFESP]; Pesquero, Joao Bosco [UNIFESP]; Lopes, Jose Daniel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The existence of murine peritoneal osteoclast precursors has been already described. Also, recent reports evidenced an interplay between B lymphocytes and osteoclasts development. B-1 cells comprise a B-lymphocyte subset that resides mostly in pleural and peritoneal cavities. It has been demonstrated that B-1 cells can differentiate into mononuclear phagocytes and form multinucleated giant cells. Based on these findings, we investigated the role of B-1 lymphocytes in bone resorption and osteoclastogenesis. in vivo experimental periodontitis induced in B-1 deficient Xid mice demonstrated that bone resorption is impaired in these animals. However, reconstitution of Xid mice with B-1 cells increased bone resorption to near Balb/c values. B-1 cell derived phagocytes express the receptor activator of nuclear factor-kappa B (RANK) and the macrophage colony-stimulating factor receptor (M-CSFR). When cultured with RANK-ligand (RANKL) and M-CSF, B-1 cells became tartrate resistant acid phosphatase (TRAP) positive multinucleated cells, a typical osteoclast phenotype. Lacunae formation was observed when cells were cultivated onto a calcium phosphate analog, indicating functional differentiation of B1 cells into osteoclast-like cells. the dynamics of their IgM expression showed that this lymphoid marker was downregulated along the differentiation of B-1 lymphocytes into osteoclasts. Our results unveiled the first evidence that B-1 cells have a role in osteoclastogenesis and bone resorption and offer new insights in the relationship between bone and lymphoid cells. (C) 2011 Elsevier GmbH. All rights reserved.
- ItemSomente MetadadadosEffects of Short-Term Risedronate on Bone Resorption and Patient Satisfaction in Postmenopausal Osteoporosis Patients(Elsevier B.V., 2009-01-01) Fernandes, Cesar E.; Zerbini, Cristiano; Russo, Luis A.; Albernaz, Marco A.; Eis, Sergi R.; Szejnfeld, Vera L. [UNIFESP]; Pompei, Luciano M.; Fac Med ABC; Hosp Heliopolis São Paulo; CCBR Brasil Ctr Anal & Pesquisas Clin; Hosp Materno Infantil Goiania; Ctr Diagnost & Pesquisa Osteoporose Espirito Sant; Universidade Federal de São Paulo (UNIFESP)This multicenter. open-label study evaluated the effects of short-term risedronate on bone resorption and patient satisfaction in postmenopausal women with osteoporosis in Brazil. Entry requirements included: osteoporosis of the spine/femoral neck diagnosed by a bone mineral density (BMD) T-score <= -2.5 or radiographic fragility fracture within the last year and no treatment with osteoporosis medication in the preceding 3 mo. Patients were treated with once weekly risedronate of 35 mg for 12 wk. Patients also received 1000 mg calcium carbonate and 400 IU vitamin D. the main outcome was the effect on bone resorption, as assessed by the quantification of serum C-telopeptide of type I collagen (CTX). of the 556 women screened, 480 women received >= 1 dose of study drug (intent-to-treat [ITT] population), and 390 completed treatment (81%). After 12 wk, CTX decreased in 94% of patients (from 0.419 +/- 0.234 to 0.158 +/- 0.171 mu g/L, p < 0.0001). Mean CTX reduction was 60.6%. Patient satisfaction was good/excellent in 91.7% of patients. A total of 156 adverse events (AEs) were reported by 113 (23.5%) patients in the ITT population. Digestive symptoms emerged or worsened in 7.1% and 3.5%. respectively. Five patients (1.0%) experienced serious AEs, not considered to be related to risedronate. in conclusion, risedronate significantly reduced scrum CTX after 12-wk treatment. Almost all patients reported good/excellent satisfaction.
- ItemAcesso aberto (Open Access)Movimentação dentária experimental em murinos: período de observação e plano dos cortes microscópicos(Dental Press Editora, 2009-02-01) Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Santamaria Jr, Milton [UNIFESP]; Consolaro, Maria Fernanda Martins-ortiz; Consolaro, Alberto; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)AIM: This study aims to elucidate the relevant microscopic aspects of induced tooth movement in murines with regard to: (1) different study periods; and (2) transverse and longitudinal directions of microscopic sections. Experimental studies on induced tooth movement in murines use variable study periods and directions of microscopic sections, including those studies that specifically use the model adopted by Heller and Nanda in 1979. This manuscript was prepared in order to contribute to: (1) selection of the best study design for future studies on induced tooth movement in murines, and (2) improve the analysis criteria to be used by other investigators. METHODS: The study was conducted on 50 male Wistar rats with 90 days of age, submitted to induced tooth movement for periods of 3, 5, 7 and 9 days. The maxillary left first molar was submitted to mesial inclination by application of 75cN of force. Qualitative microscopic analysis evaluated the tissue and cellular phenomena secondary to induced tooth movement, at the different study periods and on transverse and longitudinal sections. RESULTS: Among the phenomena investigated, hyaline areas of periodontal ligament were mostly observed at 5 days, and root resorptions were remarkable and well delineated at 9 days. Both phenomena affected mainly the distal roots, especially the distobuccal root. CONCLUSION: Considering the present objective, study periods of 5 to 9 days and transverse microscopic sections may be suggested for future studies on this subject.