Navegando por Palavras-chave "Bladder"

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    Análise da expressão dos receptores envolvidos no controle vesical em pacientes com bexiga desfuncionalizada e do comportamento miccional após o transplante renal
    (Universidade Federal de São Paulo (UNIFESP), 2017-11-09) Neves Neto, João Ferreira [UNIFESP]; Mesquita, Roberto Andre Soler [UNIFESP]; http://lattes.cnpq.br/9038872306641159; http://lattes.cnpq.br/0556885434249812; Universidade Federal de São Paulo (UNIFESP)
    Objective: End-stage renal disease (ESRD) patients undergoing dialysis may develop anuria. Long periods of anuria lead to interruption of the physiological storage and voiding cycle, a condition known as defunctionalized bladder (DB). The objective of this study is to evaluate the expression of bladder receptors in patients with DB and to assess voiding behavior after refunctionalization. Methods: A total of 68 patients with ESRD undergoing dialysis who were candidates to kidney transplant were divided in two groups: DB (diuresis < 300 mL/24 h; n=33) and NDB (non-DB; diuresis  300 mL/24 h; n=35). During kidney transplant a sample of the already dissected mucosa and detrusor at the site of the future ureteral implantation were collected. The expression of the following receptors was evaluated by real-time polymerase chain reaction (RT-PCR) in the mucosa and detrusor: M2, M3, 1D, 3, P2X1, P2X2, P2X3, TRPV1, TRPV4, TRPA1 and TRPM8. At 3, 6 and 12 months after kidney transplant patients answered IPSS and ICIQ-OAB questionnaires. They also filled a 3-day 24 h frequency/volume chart at 6 and 12 months. Results: There was no difference in sex and age between DB and NDB groups. Lower diuresis volume in 24 h and longer pretransplant dialysis duration was observed in DB patients. The expression of all receptors in the mucosa and in the detrusor was similar in both groups, except from 1D, which was overexpressed in the detrusor of DB relatively to NDB group. TRPM8 expression was not demonstrated in any bladder layer of any group. ICIQ-OAB symptom score was similar between the groups at 3, 6 and 12 months. There was a reduction of this score in both groups throughout the follow-up. The same pattern was found for IPSS score. Bother scores were similar between groups. No difference was observed for all parameters extracted from the frequency-volume charts between DB and NDB patients. Conclusion: Gene expression of bladder receptors involved in micturition control was similar in patients with or without DB. Bladder behavior had a similar pattern independently of pretransplant residual diuresis. These findings question the relevance of the term DB in pretransplant patients.
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    Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall
    (Springer, 2010-06-01) Almeida, Fernando Goncalves [UNIFESP]; Dantas Nobre, Yuri Tulio [UNIFESP]; Leite, Katia R.; Bruschini, Homero; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    A study was carried out to evaluate the feasibility of autologous adipose derived stem cells (ADSC) transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration.Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction (SVF). the SVF were platted to isolate ADSC. Before urethral injection, cells were labeled with DiI marker. the urethra wall was injected with 1 x 10(7) autologous cells or saline (sham). the urethra was harvested at 2, 4, and 8 weeks to identify DiI-labeled cells.At 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. At 8 weeks, the ADSCs spread and integrated with the urethra wall from the initial injection site.This is the first study to demonstrate a successful autologous ADSCs transplantation. It confirms that ADSCs can survive and integrate within the urethral wall.
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    Estrogen replacement avoids the decrease of bladder innervations in ovariectomized adult virgin rats: in vivo stereological study
    (Springer, 2009-05-01) Fraga, Rogerio de; Palma, Paulo; Dambros, Miriam [UNIFESP]; Riccetto, Cassio L. Z.; Mandarim-de-Lacerda, Carlos; Miyaoka, Ricardo; Univ Fed Parana; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de São Paulo (UNIFESP); Universidade do Estado do Rio de Janeiro (UERJ)
    The authors quantified the nerve fibers in the bladder wall of ovariectomized rats with and without estradiol replacement.This study was conducted on 40 Wistar rats (3 months old). Group 1: remained intact; Group 2: underwent bilateral ovariectomy, and after 30 days was started on subcutaneous sesame oil replacement (0.2 ml per day) for 90 days; Group 3: sham-operated, and after 30 days was started on subcutaneous sesame oil replacement (0.2 ml per day) for 90 days; Group 4: bilateral ovariectomy, and after 30 days was started on subcutaneous injection of 17 beta-estradiol (10 mu g/kg body weight) for 90 days. S-100 was used to stain nerves myelinized fibers on paraffin rat bladder sections. the G-50 grid system was used to quantitatively analyze the fibers.Long-term estrogen deprivation caused significant changes in bladder innervations, which can be characterized by a decreased number of nerve fibers by 65% (p < 0.001).
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    Future Direction in Pharmacotherapy for Non-neurogenic Male Lower Urinary Tract Symptoms
    (Elsevier B.V., 2013-10-01) Soler, Roberto [UNIFESP]; Andersson, Karl-Erik; Chancellor, Michael B.; Chapple, Christopher R.; Groat, William C. de; Drake, Marcus J.; Gratzke, Christian; Lee, Richard; Cruz, Francisco; Universidade Federal de São Paulo (UNIFESP); Hosp Israelita Albert Einstein; Wake Forest Univ; Wake Forest Baptist Med Ctr; Oakland Univ; Sheffield Teaching Hosp NHS Fdn Trust; Univ Pittsburgh; Univ Bristol; Ludwig Maximilians Univ Munchen; James Buchanan Brady Fdn; Univ Porto
    Background: the pathophysiology of male lower urinary tract symptoms (LUTS) is highly complex and multifactorial. the shift in perception that LUTS are not sex or organ specific has not been followed by significant innovations regarding the available drug classes.Objective: To review pathophysiologic mechanisms and clinical and experimental data related to the development of new pharmacologic treatments for male LUTS.Evidence acquisition: the PubMed database was used to identify articles describing experimental and clinical studies of pathophysiologic mechanisms contributing to male LUTS and, supported by them, new pharmacotherapies with clinical or experimental evidence in the field.Evidence synthesis: Several pathologic processes (eg, androgen signaling, inflammation, and metabolic factors) and targets (eg, the urothelium, prostate, interstitial cells, detrusor, neurotransmitters, neuromodulators, and receptors) have been implicated in male LUTS. Some newly introduced drugs, such as phosphodiesterase type 5 inhibitors and beta 3-adrenergic agonists, have just started broad use in clinical practice. Drugs with potential benefit, such as vitamin D3 receptor analogs, gonadotropin-releasing hormone antagonists, cannabinoids, and drugs injected into the prostate, have been evaluated in experimental studies and have progressed to clinical trials. However, safety and efficacy data for these drugs are still scarce. Some compounds with interesting profiles have only been tested in experimental settings (eg, transient receptor potential channel blockers, Rho-kinase inhibitors, purinergic receptor blockers, and endothelin-converting enzyme inhibitors).Conclusions: New pathophysiologic mechanisms of male LUTS are described that lead to the continuous development of new pharmacotherapies. To date, few drugs have been added to the current armamentarium, and several are in various phases of clinical or experimental investigation. (C) 2013 European Association of Urology. Published by Elsevier B.V. All rights reserved.