Navegando por Palavras-chave "Autologous"

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    Assessment of surgical outcomes of limbal transplantation using simple limbal epithelial transplantation technique in patients with total unilateral limbal deficiency
    (Consel Brasil Oftalmologia, 2016) Queiroz, Ana Gabriela [UNIFESP]; Oiticica Barbosa, Martina Maria [UNIFESP]; Santos, Myrna Serapiao [UNIFESP]; Barreiro, Telma Pereira [UNIFESP]; Pereira Gomes, Jose Alvaro [UNIFESP]
    This study aimed to evaluate the effectiveness of the novel simple limbal epithelial transplantation (SLET) technique, which reduces the risk of iatrogenic limbal stem cell deficiency in the donor eye. Four patients with total unilateral limbal stem cell deficiency received a limbal graft, measuring 4 mm x 2 mm, from the contralateral healthy eye in a single surgical procedure. The graft was divided into 10-20 pieces and distributed on the corneal surface. At 6-month follow-up, a completely avascular corneal epithelial surface was obtained in two patients, and there was improvement in visual acuity in one patient. The limbal grafts did not adhere to the cornea in one patient. No serious complications related to the surgery were observed in this study.
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    N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Amorim, Elivânia Marques Gomes de; Damous, Luciana Lamarão [UNIFESP]; Durando, Maria Clara Silva; Saraiva, Márcia Viviane Alves; Koike, Marcia Kiyomi; Montero, Edna Frasson de Souza [UNIFESP]; Federal University do Vale do São Francisco; Universidade Federal de São Paulo (UNIFESP); University of São Paulo Medical School Obstetrics and Gynecology Department; Federal University of Ceara Faculty of Medicine; Universidade de São Paulo (USP)
    PURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC.METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test.RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation.CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.