Navegando por Palavras-chave "Apple extract"

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    Chemopreventive activity of apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide
    (Elsevier B.V., 2014-08-01) Ribeiro, Flavia Andressa Pidone [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; Gollucke, Andrea Pittelli Boiago; Ferreira, Monica Siqueira; Catharino, Rodrigo Ramos; Aguiar, Odair [UNIFESP]; Spadari-Bratfisch, Regina Celia [UNIFESP]; Barbisan, Luis Fernando; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Catholic Santos Univ; Universidade Estadual de Campinas (UNICAMP); Universidade de São Paulo (USP)
    Objective: the aim of this study was to evaluate the chemopreventive activity of an apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide (4NQO).Methods: A total of 30 male Wistar rats were distributed into five groups as follows (n = 6 per group): Group 1, negative control group (non-treated group); Group 2, received 4NQO during 8 weeks in drinking water and treated with apple extract at 1% by gavage between the first and fourth weeks daily (initiation phase); Group 3, received 4NQO for 8 weeks in drinking water and treated with apple extract by gavage at 1% between the fifth and eighth weeks daily (promotion phase); Group 4, received apple extract at 1% by gavage for 8 consecutive weeks only; and Group 5, received 4NQO for 8 weeks in drinking water daily.Results: Histopathological analysis revealed decreased hyperplasic lesions in Group 2 when compared with Group 5. Likewise, decreased dysplastic lesions in Group 3 were observed when compared with Group 5. in Groups 2 and 3, decreased COX-2 and TNF-alpha gene expressions were observed when compared with Group 5. Cytochrome c and caspase 3 levels increased in Groups 2 and 3 when compared with Group 5.Conclusion: in conclusion, our results demonstrate that apple extract suppresses rat tongue carcinogenesis as a result of anti-inflammatory activity and apoptosis through the intrinsic mitochondrial pathway. (C) 2014 Elsevier B.V. All rights reserved.
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    Effect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Acid
    (Cellular & Molecular Biology Research Center, 2017) Pastrelo, Maurcio Mercaldi [UNIFESP]; Ribeiro, Carla Caroline Dias [UNIFESP]; Duarte, Joselmo Willamys [UNIFESP]; Gollucke, Andrea Pittelli Boiago [UNIFESP]; Artigiani Neto, Ricardo [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Miszputen, Sender Jankiel [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Paiotti, Ana Paula Ribeiro [UNIFESP]
    Reactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1-Sham/saline solution, G2-CAE/control, G3-acetic acid/control, G4-curative-CAE treatment and G5-preventive-CAE treatment. Eight days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8-hydroxy-20-deoxyguanosine (8-OHdG) via immunohistochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD.