Navegando por Palavras-chave "Antioxidant effects"

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    Eicosapentaenoic acid and docosahexaenoic acid exert anti-inflammatory and antinociceptive effects in rodents at low doses
    (Elsevier B.V., 2013-05-01) Pereira Nobre, Maria Elizabeth [UNIFESP]; Correia, Alyne Oliveira; Borges, Marilia de Brito; Araujo Sampaio, Thayga Maria; Chakraborty, Solon Arcoverde; Goncalves, Danilo de Oliveira; Castro Brito, Gerly Anne de; Almeida Moreira Leal, Luzia Kalyne; Bezerra Felipe, Cicero Francisco; Lucetti, Daniel Luna; Arida, Ricardo Mario [UNIFESP]; Barros Viana, Glauce Socorro de; Fac Med Estado Juazeiro do Norte FMJ; Universidade Federal de São Paulo (UNIFESP); Fed Univ Ceara UFC
    In the present study, we evaluated omega-3 polyunsaturated fatty acid (PUPA) (consisting of 20:5n-3 and 22:6n-3) properties on inflammation and nociception. Among the in vivo tests, writhing, formalin, and hot plate tests were conducted in mice, and carrageenan-induced paw edema, peritonitis, and Hargreaves tests were performed in rats. Following the carrageenan-induced edema, immunohistochemistry for tumor necrosis factor-alpha (TNF-alpha) was also carried out. We found that omega-3 PUPA treatment significantly decreased acetic acid-induced abdominal contortions as well as the first and second phases of the formalin test, which were reversed by naloxone. the carrageenan-induced rat paw edema was significantly reduced, along with neutrophil migration to the peritoneal cavity in the omega-3 PUPA treatment. in addition, there was a decrease in TNF-a immunostained cells in the inflamed paw with the omega-3 treatment compared with no omega-3. Withdrawal threshold in response to the thermal stimulation was significantly increased by the omega-3 treatment in the Hargreaves and hot plate tests. the in vitro studies (myeloperoxidase, lactate dehydrogenase, MTT cell viability and lipid peroxidation assays) were performed in human neutrophils. These studies showed that omega-3 treatment significantly decreased myeloperoxidase release, presented no cytotoxicity, and did not alter lipid peroxidation. Our study suggests that omega-3 PUPA anti-inflammatory and antinociceptive actions may involve inhibition of cyclooxygenases and microglial activation, leading to a reduced release of proinflammatory cytokines such as TNF-alpha, among other factors. the omega-3 PUFAs are potential candidates used alone or in combination with conventional nonsteroidal anti-inflammatory drugs, for the treatment of diseases where inflammation plays an important role. (C) 2013 Elsevier Inc. All rights reserved.
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    Schinus terebinthifolius administration prevented behavioral and biochemical alterations in a rotenone model of Parkinson's disease
    (Soc Brasileira Farmacognosia, 2016) Sereniki, Adriana; Linard-Medeiros, Cybelle F. B.; Silva, Shirliane N.; Silva, Juciene B. R.; Peixoto Sobrinho, Tadeu J. S.; da Silva, Juliano R.; Alves, Lariza D. S.; Smaili, Soraya S. [UNIFESP]; Wanderley, Almir G.; Lafayette, Simone S. L.
    Parkinson's disease is a neurodegenerative disorder characterized by motor impairment, cognitive decline and psychiatric symptoms. Schinus terebinthifolius Raddi, Anacardiaceae, had been studied for its anti-inflammatory and antioxidant properties, and in this study, the stem bark was evaluated for the neuroprotective effects on behavioral and biochemical alterations induced by administrations of rotenone in rats. Behavioral evaluations were performed using open-field and rotarod. The in vitro and in vivo antioxidant activities were determined by the DPPH radical scavenging activity and lipid peroxidation method respectively. The administration of rotenone (3 mg/kg, s.c.) produced hypolocomotion, increase of immobility and muscle incoordination, while the treatment with S. terebinthifolius stem bark extract (150, 300 and 600 mg/kg p.o.) for seven days prevented rotenone-induced dysfunctional behavior. Biochemical analysis of the substantia nigra, striatum and cortex revealed that rotenone administration significantly increased lipid peroxidation, which was inhibited by treatment with all doses of S. terebinthifolius. The results suggested neuroprotective effect of S. terebinthifolius possibly mediated through its antioxidant activity, indicating a potential therapeutic benefit of this species in the treatment of Parkinson's disease. (C) 2015 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.