Navegando por Palavras-chave "Antimicrobial Susceptibility Methods"

Agora exibindo 1 - 1 de 1
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Influência dos Métodos de Sensibilidade aos Antimicrobianos no Uso Clínico das Polimixinas
    (Universidade Federal de São Paulo (UNIFESP), 2010-05-04) Silva, Itacy Gonçalves de Siqueira e [UNIFESP]; Gales, Ana Cristina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Acinetobacter spp. e Pseudomonas aeruginosa are important pathogens that cause infections in Brazilian hospitals and have become resistant to almost every available antimicrobial. Thereby, the clinical indication of parenteral use of polymyxin has been reestablished in recent years. Therefore, the clinical laboratory of microbiology need be able to perform reliable susceptibility antimicrobial tests. Objective: Analyze the influence of susceptibility antimicrobial tests in the clinical use of polymyxins. Material and Methods: Nine P. aeruginosa and 10 Acinetobacter spp. strains were tested to polymyxin susceptibility. The results of agar dilution, broth microdilution and Etest were compared, according CSLI. The influence of culture medium, calcium concentration, inoculum concentration and incubation time in the susceptibility antimicrobial test was evaluated. Results: There was good agreement between different polymyxin antimicrobial susceptibility methods for Acinetobacter spp isolates, but not for P. aeruginosa. It was observed 100% of agreement between Iso-sensitest and Müeller-Hinton medium through agar dilution and Etest for Acinetobacter spp. isolates. Through broth microdilution occurred 90% of agreement for polymyxin B and 60% for colistin. Between P. aeruginosa isolates, Etest had greater MIC variation when using different culture mediuns. In general, BHI medium had low MICs comparing with Müller- Hinton. The calcium concentration increase in the culture medium promoted MICs elevation in ±1Log2 of dilution, for both microrganisms. Etest had 55,6% (polymyxin B) and 88,9% (colistin) of minor error, and 11,1% of major error in polymyxin B. Inoculum size had greater influence in agar dilution, with minor error rates of 10% (polymyxin B) and 30% (colistin) for Acinetobacter spp. isolates, and 33,3% (polymyxin B) and 66,6% (colistin) for P. aeruginosa isolates. Different time incubations caused MICs variation only between P. aeruginosa isolates. Etest method had 33,3% (polymyxin B) and 44,4%(colistin) of minor error. Through agar dilution, the minor error rate was 11,1% with incubation time variation.