Navegando por Palavras-chave "Animal-Models"

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    Diphenyl diselenide attenuates oxidative stress and inflammatory parameters in ulcerative colitis: a comparison with ebselen
    (Funpec-Editora, 2016) Petronilho, Fabricia; Michels, Monique; Danielski, Lucineia G.; Goldim, Mariana Pereira; Florentino, Drielly; Vieira, Andriele; Mendonca, Mariana G.; Tournier, Moema; Piacentini, Barbara; Della Giustina, Amanda; Leffa, Daniela D.; Pereira, Gregorio W. [UNIFESP]; Pereira, Volnei D.; Teixeira Da Rocha, Joao Batista
    Objetive: The aim of this study was to evaluate the effects of diphenyl diselenide (PhSe)(2) and ebselen (EB) in ulcerative colitis (UC) induced by dextran sulfate sodium (DSS) in rats. Methods: The effects of (PhSe)(2) and EB in rats submitted to DSS-induced colitis were determined by measurement of oxidative stress parameters, inflammatory response and bowel histopathological alterations. Results: Animals developed moderate to severe neutrophil infiltration in histopathology assay in DSS rats and (PhSe)(2) improved this response. Moreover, the treatment with (PhSe)(2) decreased the oxidative damage in lipids and proteins, as well as reversed the superoxide dismutase (SOD) and catalase (CAT) levels in rats treated with DSS. LB was able only to reverse damage in lipids and the low levels of SOD in this animal model. Conclusions: The organoselenium compounds tested demonstrated an anti-inflammatory and antioxidant activity reducing the colon damage, being (PhSe)(2) more effective than EB. (C) 2016 Elsevier GmbH. All rights reserved.
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    The plant-derived bauhinia bauhinioides kallikrein proteinase inhibitor (rbbki) attenuates elastase-induced emphysema in mice
    (Acta Cirurgica Brasileira, 2016) Martins-Olivera, Bruno Tadeu; Almeida-Reis, Rafael; Theodoro-Junior, Osmar Aparecido; Oliva, Leandro Vilela; dos Santos Nunes, Natalia Neto [UNIFESP]; Olivo, Clarice Rosa; de Brito, Marlon Vilela [UNIFESP]; Prado, Carla Maximo [UNIFESP]; Leick, Edna Aparecida; Martins, Milton de Arruda; Vilela Oliva, Maria Luiza [UNIFESP]; Righetti, Renato Fraga; Lopes Calvo Tiberio, Iolanda de Fatima
    Background. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2 alpha, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment.