Navegando por Palavras-chave "Allopurinol"
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- ItemAcesso aberto (Open Access)Efeito da ventilação com diferentes frações inspiradas de oxigênio e do alopurinol na isquemia-reperfusão pulmonar em ratos(Colégio Brasileiro de Cirurgiões, 2004-10-01) Silva, Fábio May Da [UNIFESP]; Silveira, Rafael José; Hallal, Ana Luiza De Lima Curi; Wilhelm Filho, Danilo; Cardoso, João José De Deus; Leao, Luiz Eduardo Villaca [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Secretaria Estadual de Saúde de Santa Catarina; Universidade Federal de Santa Catarina; Faculdade de Saúde Pública e Epidemiologista; Universidade Federal de Santa Catarina Departamento de Ecologia e Zoologia; Universidade Federal de Santa Catarina Departamento de Clínica CirúrgicaBACKGROUND: To evaluate the effects on lung ischaemia-reperfusion injury of ventilation with inspired oxygen concentrations of 0.21 and 1.00, and of allopurinol (an antioxidant). METHODS: Sixty Wistar rats were randomly allocated to six groups. Group I was the control group, while Groups II and III comprised the animals ventilated during ischaemia-reperfusion with 0.21 and 1.00 FiO2, respectively. The three remaining groups IA, IIA and IIIA were pre-treated with 100 mg/kg allopurinol and submitted to similar procedures as groups I, II and III respectively. The model used was normothermic ischaemia-reperfusion in situ. The duration of ischaemia was 30 minutes and that of reperfusion 10 minutes. Outcome measures included mean systemic arterial pressure (MAP), the partial pressure of oxygen/inspired fraction of oxygen (PaO2/FiO2) ratio, the level of thiobarbituric acid reactive substances (TBARS) in the lung tissue and the lung wet/dry weight ratio. RESULTS: There was a significant (p<0.05) decrease in the MAP among groups III x I, II x IIA and III x IIIA. With regard to the PaO2/FiO2 there was a significant (p<0.05) decrease between groups III x II and III x IIIA. The TBARS level showed a significant (p<0.05) reduction in group III compared to group IIIA. Meanwhile, there was a significant (p<0.05) increase in the lung wet/dry weight ratio comparing groups III x II, II x IIA and III x IIIA. CONCLUSION: Ventilation with 21% oxygen (FiO2=0.21), when compared to ventilation with 100% oxygen (FiO2 =1.0), resulted in a smaller decrease in MAP, an improved PaO2/FiO2 ratio and less pulmonary oedema. Compared to non-treated groups, the pre-treatement with allopurinol lead to a lower MAP decrease, an improved PaO2/FiO2 ratio, lower TBARS production and less pulmonary oedema.
- ItemAcesso aberto (Open Access)Efeito do alopurinol sobre a morfologia do testículo do rato submetido a isquemia, pela torção do cordão espermático seguida de reperfusão(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2005-12-01) Antoniolli, Andréia Conceição Milan Brochado [UNIFESP]; Ortiz, Valdemar [UNIFESP]; Silva, Roberto Antoniolli Da [UNIFESP]; Tognini, João Ricardo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); universidade Federal de Mato Grosso de Sul departamento de cirurgiaPURPOSE: In this investigation has been studied the effect of the alopurinol on the reperfusion injury phenomenon in testicles of rats. METHODS: Eighty adults Wistar rats were divided in 4 groups: sham without alopurinol, sham with alopurinol, rigth testicular torsion for one hour without alopurinol and rigth testicular torsion for one hour with alopurinol. Bilateral orchiectomy was performed 60 days of the experiment and the testis were precessed for optical microscopy, and edamined to asses the seminiferous ducts and the spermatogenic cells. RESULTS: There was significant atrophy and histological damage of the rigth testis. CONCLUSION: Alopurinol did not protected the testis against the effects of the reperfusion injury.
- ItemAcesso aberto (Open Access)Efeito dos inibidores da xantina oxisidase em ratos submetidos à sepse induzida por LPS(Universidade Federal de São Paulo (UNIFESP), 2017-04-28) Ramos, Maria Fatima de Paula [UNIFESP]; Schor, Nestor [UNIFESP]; http://lattes.cnpq.br/8276708741672261; http://lattes.cnpq.br/5990424588159711; Universidade Federal de São Paulo (UNIFESP)Objectives: To evaluate the effect of xanthine oxidase inhibitors in rats submitted to LPS administration. Material and Methods: LPS was administered i.p. (10 mg/Kg) every 24 hours until the 3rd day. XOi were given by gavage each 24 hour for 3 days. Allopurinol (Alo, 2 mg/kg) and febuxostat (Feb, 1 mg /kg) were used in an equivalent dose as in clinical use. To increase uric acid (UrAc) levels, Oxonic Acid (Oxo) was administered by gavage (750mg/Kg/day) during 5 days. UrAc measurements were done at baseline and at 6th day. The animals were divided into 10 groups (n = 6 each): 1-Control, 2-Alo, 3-Feb, 4-LPS, 5-LPSAlo, 6-LPSFeb, 7-Oxo, 8-OxoLPS, 9-OxoLPSAlo and 10-OxoLPSFeb. Data were evaluated by ANOVA and survival curves (Chi square) considering the value of p<0.05 for statistical significance. Data were reported as mean and standard deviation. Results: All animals that received LPS developed renal function impairment. Immuno histochemistry staining technique analysis in this groups, revealed less cellular proliferation evaluate by PCNA and increased apoptosis measured by cleaved caspase-3, compatible with severe sepsis. With simultaneous administration of Alo, in this experimental sepsis induced by LPS, a higher impact of septic shock was noted with significant increase in mortality rates (p<0.05) in the LPSAlo group (28/34; 82%) when compared with LPS group (10/16; 63%) and LPSFeb group (11/17; 65%). We noted lower mean values of creatinine clearance in the group who received only LPS (44%) (p<0.05); in the LPSAlo group (60%) (p<0.01) and in the LPSFeb group (35%)(p<0.05). Additionally, we obtained higher levels of reactive oxygen species (nMol/mg of creatinine in the 24-hour urine test, p<0.001) in the groups that received Alo, in LPSAlo group (293.3±35.8) and OxoLPSAlo group (304.3±60.4) compared with LPS group (267.8±44.9), LPSFeb group (220.0±39.1), OxoLPS group (236.7±33.2) and OxoLPSFeb group (211.2±43.6). In all the groups who received LPS, the levels of TNF-α increased about 3 times in relation to control groups in 17 hours and returned to basal levels in all groups, except in the LPSAlo and OxoLPSAlo groups where this late decline was not observed. We obtained significant superior values of IL-6 (pg/mL) in the groups that received Alo, LPSAlo group (238.8±28) (p<0.05) and OxoLPSAlo group (275.7±52) (p<0.01) compared with LPS group (175.7±40), LPSFeb group (199.5±23), but there was no statistically significant difference between the OxoLPS (231.3±19 pg/mL) e OxoLPSFeb (229.2±30 pg/mL) or LPSAlo vs OxoLPSAlo groups. After 72 hours, lower IL-6 values were present in about 50% of cases in all groups, except in LPSAlo and OxoLPSAlo groups were these values remained higher, both about 15 times in relation to the control group. Conclusion: In this study, simultaneous administration of Alo in the experimental sepsis induced by LPS enhanced the septic shock with increased in mortality, renal function impairment, high values for oxygen reactives species and for interleukins pro-inflammatory. Differently, when we administrate febuxostat, we do not observed potentiation in the septic situation, probably by higher selectivity and no interference in another metabolic event that probably occur with allopurinol.
- ItemSomente MetadadadosInfections in heart transplant recipients in Brazil: the challenge of Chagas' disease(Elsevier B.V., 2010-03-01) Godoy, Henrique Luiz dos de [UNIFESP]; Guerra, Carla Morales [UNIFESP]; Viegas, Ruy Felipe Melo [UNIFESP]; Diniz, Rosiane Viana Zuza [UNIFESP]; Branco, João Nelson Rodrigues [UNIFESP]; Amato-Neto, Vicente; Almeida, Dirceu Rodrigues de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)BACKGROUND: Despite the high incidence of infections after heart transplantation, there is limited information about its epidemiology in patients from countries where Chagas' disease is endemic.METHODS: We analyzed the occurrence of infections in 126 patients aged older than 18 years who underwent transplantation from 1986 through 2007 at a Brazilian University Hospital and who survived at least 48 hours.RESULTS: Heart failure diagnoses before transplantation were idiopathic dilated cardiomyopathy (38.6%), Chagas' disease (34.9%), coronary artery disease (19.8%), and others (6.3%). the respiratory tract was the most common site of infections (40.9%), followed by surgical wound site (18.1%). Trypanosoma cruzi reactivations occurred in, 38.8% of Chagas' disease patients: 47.0% had myocarditis, 23.5% had skin lesions, and 29.4% had both. New-onset ventricular dysfunction was observed in 47.0%, with complete response after specific treatment, and 41.0% were asymptomatic cases, diagnosed by routine endomyocardial biopsies. No patient died from such events. No differences in survival were found after 5 years of follow-up between recipients with and without Chagas' disease (p = 0.231).CONCLUSIONS: in a heart transplant population from a developing country, infectious complications occurred at a high rate. Tropical illnesses were uncommon, except for the high rate of Chagas' disease reactivations. Despite that, the overall outcome of these patients was similar to that of recipients with other cardiomyopathies. J Heart Lung Transplant 2010;29:286-90 (C) 2010 International Society for Heart and Lung Transplantation. All rights reserved.