Navegando por Palavras-chave "Aerobic exercise training"

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    Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats
    (Springer, 2015-04-01) Andrade, Luiz Henrique Soares de [UNIFESP]; Moraes, Wilson Max Almeida Monteiro de [UNIFESP]; Matsuo Junior, Eduardo Hiroshi [UNIFESP]; Moura, Elizabeth de Orleans Carvalho de [UNIFESP]; Antunes, Hanna Karen Moreira [UNIFESP]; Montemor, Jairo [UNIFESP]; Antonio, Ednei Luiz [UNIFESP]; Bocalini, Danilo Sales [UNIFESP]; Serra, Andrey Jorge [UNIFESP]; Tucci, Paulo José Ferreira [UNIFESP]; Brum, Patricia Chakur; Medeiros, Alessandra [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Sao Judas Tadeu; Univ Nove Julho; Universidade de São Paulo (USP)
    The activity of the ubiquitin proteasome system (UPS) and the level of oxidative stress contribute to the transition from compensated cardiac hypertrophy to heart failure in hypertension. Moreover, aerobic exercise training (AET) is an important therapy for the treatment of hypertension, but its effects on the UPS are not completely known. the aim of this study was to evaluate the effect of AET on UPS's activity and oxidative stress level in heart of spontaneously hypertensive rats (SHR). A total of 53 Wi-star and SHR rats were randomly divided into sedentary and trained groups. the AET protocol was 59/week in treadmill for 13 weeks. Exercise tolerance test, non-invasive blood pressure measurement, echocardiographic analyses, and left ventricle hemodynamics were performed during experimental period. the expression of ubiquitinated proteins, 4-hydroxynonenal (4-HNE), Akt, phospho-Akt(ser473), GSK3 beta, and phospho-GSK3 beta(ser9) were analyzed by western blotting. the evaluation of lipid hydroperoxide concentration was performed using the xylenol orange method, and the proteasomal chymotrypsin-like activity was measured by fluorimetric assay. Sedentary hypertensive group presented cardiac hypertrophy, unaltered expression of total Akt, phospho-Akt, total GSK3 beta and phospho-GSK3 beta, UPS hyperactivity, increased lipid hydroperoxidation as well as elevated expression of 4-HNE but normal cardiac function. in contrast, AET significantly increased exercise tolerance, decreased resting systolic blood pressure and heart rate in hypertensive animals. in addition, the AET increased phospho-Akt expression, decreased phospho-GSK3 beta, and did not alter the expression of total Akt, total GSK3 beta, and ubiquitinated proteins, however, significantly attenuated 4-HNE levels, lipid hydroperoxidation, and UPS's activity toward normotensive group levels. Our results provide evidence for the main effect of AET on attenuating cardiac ubiquitin proteasome hyperactivity and oxidative stress in SHR rats.
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    Aerobic exercise training rescues protein quality control disruption on white skeletal muscle induced by chronic kidney disease in rats
    (Wiley, 2018) Moraes, Wilson Max Almeida Monteiro de [UNIFESP]; Souza, Pamella Ramona Moraes de; Paixao, Nathalie Alves de; Sousa, Luis Gustavo Oliveira de; Ribeiro, Daniel Araki [UNIFESP]; Marshall, Andrea G.; Prestes, Jonato; Irigoyen, Maria Claudia; Brum, Patricia Chakur; Medeiros, Alessandra [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    We tested whether aerobic exercise training (AET) would modulate the skeletal muscle protein quality control (PQC) in a model of chronic kidney disease (CKD) in rats. Adult Wistar rats were evaluated in four groups: control (CS) or trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise (60 min., five times/wk for 2 months). We evaluated motor performance (tolerance to exercise on the treadmill and rotarod), cross-sectional area (CSA), gene and protein levels related to the unfolded protein response (UPR), protein synthesis/survive and apoptosis signalling, accumulated misfolded proteins, chymotrypsin-like proteasome activity (UPS activity), redox balance and heat-shock protein (HSP) levels in the tibialis anterior. 5/6NxS presented a trend towards to atrophy, with a reduction in motor performance, down-regulation of protein synthesis and up-regulation of apoptosis signalling; increases in UPS activity, misfolded proteins, GRP78, derlin, HSP27 and HSP70 protein levels, ATF4 and GRP78 genes; and increase in oxidative damage compared to CS group. In 5/6NxE, we observed a restoration in exercise tolerance, accumulated misfolded proteins, UPS activity, protein synthesis/apoptosis signalling, derlin, HSPs protein levels as well as increase in ATF4, GRP78 genes and ATF6α protein levels accompanied by a decrease in oxidative damage and increased catalase and glutathione peroxidase activities. The results suggest a disruption of PQC in white muscle fibres of CKD rats previous to the atrophy. AET can rescue this disruption for the UPR, prevent accumulated misfolded proteins and reduce oxidative damage, HSPs protein levels and exercise tolerance.
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    Efeito do treinamento físico aeróbico na hipertrofia cardíaca de ratos espontaneamente hipertensos: papel do sistema ubiquitina proteassoma
    (Universidade Federal de São Paulo (UNIFESP), 2013-08-28) Andrade, Luiz Henrique Soares de [UNIFESP]; Medeiros, Alessandra [UNIFESP]; http://lattes.cnpq.br/0071198026371230; Universidade Federal de São Paulo (UNIFESP)
    Hypertension is a clinical condition that contributes to the development of cardiac hypertrophy. The activity level of the ubiquitin proteasome system (UPS) is involved in the initiation and progression of cardiac hypertrophy. Moreover, physical training can decrease blood pressure, reduce or reverse the pathological cardiac hypertrophy and this response may, at least in part, occur through changes in the activity of the SUP in the heart. The purpose of this study was to investigate the effect of aerobic exercise training in cardiac hypertrophy and activity of UPS in spontaneously hypertensive rats (SHR). For this, we used Wistar and SHR rats (8 weeks old) randomly divided into sedentary and trained, and the exercise training protocol was 5x/week in treadmill, low intensity, 60 min, for 13 weeks. Before, during and after the experimental period exercise tolerance test was performed. The recording indirect systolic blood pressure (SBP), heart rate (HR) and body weight were evaluated once a week. The proteasome activity in the catalytic site of chymotrypsin was measured by fluorimetric assay. The tr aining significantly increased exercise tolerance in hypertensive animals, decreased SBP and HR at rest after the period, and elevated diastolic function. In addition, it restored the proteasome activity on the control groups levels. Data from this study demonstrated the important non-pharmacological therapeutic effect of exercise on ubiquitin proteasome system in hypertension.
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    Efeitos do treinamento físico aeróbio sobre a resposta à proteína mal enovelada na musculatura esquelética de ratos submetidos à uremia experimental
    (Universidade Federal de São Paulo (UNIFESP), 2016-07-28) Moraes, Wilson Max Almeida Monteiro de [UNIFESP]; Medeiros, Alessandra [UNIFESP]; http://lattes.cnpq.br/0071198026371230; Universidade Federal de São Paulo (UNIFESP)
    The present study tested whether aerobic exercise training (AET) would modulate the unfolded protein response (UPR) in a model of Chronic Kidney Disease (CKD) in rats. Adult Wistar rats were evaluated in 4 groups: control (CS), control trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise for 60 min, 5 times/wk for 2 months. We evaluated motor performance (tolerance to exercise in treadmill and rotarod), cross sectional area (AST), gene and protein levels related to UPR, protein synthesis/survive and apoptosis signaling, accumulated misfolded proteins, chymotripsin-like proteasome activity (UPS activity), redox balance and HSPs protein levels in tibialis anterior. Despite the AST were not different between groups, the 5/6NxS presented a reduction in motor performance followed by down regulation in protein synthesis and up regulation of apoptosis signaling, increased UPS activity, misfolded proteins, GRP78, derlin, HSP27 and HSP70 protein levels and ATF4 and GRP78 genes, increased in oxidative damage compared to CS group. In 5/6NxE, we observed restoration in exercise tolerance, accumulated misfolded proteins, UPS activity, protein synthesis and apoptosis signaling, derlin and HSPs protein levels as well as increased in ATF4 and GRP78 genes and GRP78,ATF6? protein levels accompanied by an decrease in oxidative damage and increased catalase and glutathione-peroxidase activities. These results suggest that an UPR is activated in white muscle fibers of CKD rats, independently of atrophy and that AET amplified this response, but prevented accumulated misfolded proteins, promoting reduction in oxidative damage, HSPs protein levels and exercise tolerance.