Navegando por Palavras-chave "Acute seizures"

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    Efeito anti-inflamatório e anticonvulsivante do Canabidiol durante duas fases do ciclo estral de ratas no modelo de crise convulsiva induzida por PTZ
    (Universidade Federal de São Paulo (UNIFESP), 2019-02-28) Janisset, Nilma do Rocio Lara de Lima [UNIFESP]; Monteiro, Beatriz de Oliveira [UNIFESP]; Romariz, Simone Amaro Alves [UNIFESP]; http://lattes.cnpq.br/4734652251203877; http://lattes.cnpq.br/0245964878412260; http://lattes.cnpq.br/4734652251203877; Universidade Federal de São Paulo (UNIFESP)
    Cannabidiol (CBD) is one of the major cannabinoids present in Cannabis sativa with great therapeutic potential. According to evidence from preclinical and clinical studies, CBD was shown to have anticonvulsive effect and has recently been proposed for the treatment of epileptic seizures. Approximately 70% of women with epilepsy face additional challenges on seizures exacerbation due to hormonal changes that occur during the menstrual cycle. Given the impact of hormonal influences on seizure activity and potential complications of treatments, the goal of the present study was to investigate the influence of two phases of the estrous cycle of female rats on the protective effect of CBD in seizures induced by pentylenetetrazole (PTZ). Wistar female rats in the estrus (E) and diestrus (D) phases were treated with vehicle (CTRL) or CBD (50mg/kg). One hour after CBD treatment, acute generalized seizures were induced by administration of PTZ (60mg/kg), and the following parameters were recorded: mortality, latency, duration and quantity of seizures. After 24h, half of animals from each group were perfused and their brains processed by immunohistochemistry for the microglial marker Iba-1, and in the other half, blood was collected for the analysis of the pro-inflammatory interleukin IL-1β levels. Behavioral parameters of seizure and inflammatory responses were analyzed and the results suggested that CBD have protective effects in estrous phase by reducing the mortality rate and quantity of seizures. Additionally, CBD in estrous phase reduced acute inflammatory responses altering the pattern of microglia expression and interleukin levels. Our findings indicated that CBD has distinct anticonvulsive effects in the two estrous cycle phases, altering the inflammatory response that occurs after acute seizures. The present results indicate that, this protective effect is influenced by hormonal variations, showing prominent effect during the estrus phase. Our study may help to clarify some issues related to the therapeutic potential of CBD in catamenial epilepsy and may contribute to the development of new therapies in the future.
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    Participation of bone marrow-derived cells in hippocampal vascularization after status epilepticus
    (W B Saunders Co Ltd, 2014-05-01) Romariz, Simone A. [UNIFESP]; Garcia, Karina de O. [UNIFESP]; Paiva, Daisylea de Souza [UNIFESP]; Bittencourt, Simone [UNIFESP]; Covolan, Luciene [UNIFESP]; Mello, Luis Eugenio [UNIFESP]; Longo, Beatriz Monteiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose: Diseases such as temporal lobe epilepsy, brain trauma and stroke can induce endothelial cell proliferation and angiogenesis in specific brain areas. During status epilepticus (SE), bone marrow-derived cells are able to infiltrate and proliferate, dramatically increasing at the site of injury. However, it is still unclear whether these cells directly participate in vascular changes induced by SE.Method: To investigate the possible role of bone marrow-derived cells in angiogenesis after seizures, we induced SE by pilocarpine injection in previously prepared chimeric mice. Mice were euthanized at 8 h, 7 d or 15 d after SE onset.Results: Our results indicated that SE modified hippocampal vascularization and induced angiogenesis. Further, bone marrow-derived GFP(+) cells penetrated through the parenchyma and participated in the formation of new vessels after SE. We detected bone marrow-derived cells closely associated with vessels in the hippocampus, increasing the density of blood vessels that had decreased immediately after pilocarpine-induced SE.Conclusion: We conclude that epileptic seizures directly affect vascularization in the hippocampus mediated by bone marrow-derived cells in a time-dependent manner. (C) 2014 British Epilepsy Association. Published by Elsevier B.V. All rights reserved.