Modelo Experimental de Radiodermatite em Coelhos
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Arquivos
Data
2015
Autores
Meirelles, Rafael Panisi de Campos [UNIFESP]
Orientadores
Ferreira, Lydia Masako [UNIFESP]
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: A tolerância cutânea à radiação é o principal fator limitante
da radioterapia. A escassez de estudos experimentais é imensa,
principalmente em animais de médio porte, como os coelhos.
Objetivo: Descrever um modelo experimental de radiodermatite em
coelhos Métodos: Neste estudo foram utilizados 24 coelhos da raça Nova
Zelândia, machos, com 6 meses de idade, peso médio de 2,5 Kg. Os
mesmos foram distribuídos em 4 grupos (n=6 para cada grupo), sendo um
grupo controle (sem nenhuma intervenção) e os demais submetidos à
radioterapia com sessão única de 10, 20 e 30 Gy, respectivamente. Foram
realizadas análises fotográficas e histopatológicas das áreas irradiadas.
Resultados: Os animais do grupo controle após 30 dias da epilação
apresentaram crescimento dos fâneros regularmente. Os animais do grupo
20 Gy e 30 Gy apresentaram alopecia com duração de 60 dias e 90 dias,
respectivamente. A partir do dia 30, a área irradiada dos animais do grupo
30 Gy apresentou grau 3 e 4 de radiodermatite, persistindo por 90 dias. A
redução progressiva da contagem de anexos, vasos sanguíneos e um
aumento da deposição de colágeno foi significante, quando comparado o
o grupo controle com o grupo de 30 Gy. Conclusão: A dosagem de 30 Gy é a mais eficaz para este modelo experimental de radiodermatite.
Introduction: The cutaneous tolerance is a limiting factor for this oncologic treatment. The lack of experimental models is enormous, primarily for mid size animals, such as rabbits. Purpose: Describe an experimental model of cutaneous radiation injury in rabbits Methods: On this study 24 six-month-old New Zealand male rabbits, with an average weight of 2.5kg, were used. They were distributed in 4 groups (n=6 per group). The control group did not receive radiotherapy and the others received one radiotherapy session of 10, 20 and 30Gy, respectively. Photographic analysis and histopathological evaluation of the irradiated areas were carried out. Results: After 30 days, the animals from the control group had all their hair grown. In spite of that, the animals from group 20Gy had a 60-day alopecia and from group 30Gy, a 90-day alopecia. After the 30th day, the 30Gy group demonstrated 90-day cutaneous radiation injuries, graded 3 and 4. A progressive reduction of glands and blood vessels count and an increase on collagen deposition was significant, when the control group is compared to the group 30Gy. Conclusion: This study demonstrates the dosage 30Gy is the most effective for this experimental model of cutaneous radiation injury in rabbits.
Introduction: The cutaneous tolerance is a limiting factor for this oncologic treatment. The lack of experimental models is enormous, primarily for mid size animals, such as rabbits. Purpose: Describe an experimental model of cutaneous radiation injury in rabbits Methods: On this study 24 six-month-old New Zealand male rabbits, with an average weight of 2.5kg, were used. They were distributed in 4 groups (n=6 per group). The control group did not receive radiotherapy and the others received one radiotherapy session of 10, 20 and 30Gy, respectively. Photographic analysis and histopathological evaluation of the irradiated areas were carried out. Results: After 30 days, the animals from the control group had all their hair grown. In spite of that, the animals from group 20Gy had a 60-day alopecia and from group 30Gy, a 90-day alopecia. After the 30th day, the 30Gy group demonstrated 90-day cutaneous radiation injuries, graded 3 and 4. A progressive reduction of glands and blood vessels count and an increase on collagen deposition was significant, when the control group is compared to the group 30Gy. Conclusion: This study demonstrates the dosage 30Gy is the most effective for this experimental model of cutaneous radiation injury in rabbits.
Descrição
Citação
MEIRELLES, Rafael Panisi de Campos. Modelo Experimental de Radiodermatite em Coelhos. 2015. 73 f. Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2015.