Using a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edema

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dc.contributor.authorCruz-Silva, Ilana [UNIFESP]
dc.contributor.authorNeuhof, Christiane
dc.contributor.authorGozzo, Andrezza Justino [UNIFESP]
dc.contributor.authorNunes, Viviane Abreu
dc.contributor.authorHirata, Izaura Yoshico [UNIFESP]
dc.contributor.authorSampaio, Misako Uemura
dc.contributor.authorFigueiredo-Ribeiro, Rita de Cassia
dc.contributor.authorNeuhof, Heinz
dc.contributor.authorAraujo, Mariana da Silva [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Giessen
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Bot
dc.date.accessioned2016-01-24T14:34:49Z
dc.date.available2016-01-24T14:34:49Z
dc.date.issued2013-12-01
dc.description.abstractAcute lung injury (ALI) is characterized by neutrophil infiltration and the release of proteases, mainly elastase (NE), cathepsin G (Cat G) and proteinase 3 (PR3), which can be controlled by specific endogenous inhibitors. However, inhibitors of these proteases have been isolated from different sources, including plants. for this study, CeEI, or Caesalpinia echinata elastase inhibitor, was purified from C. echinata (Brazil-wood) seeds after acetone fractionation, followed by ion exchange and reversed phase chromatographic steps. Characterization with SDS-PAGE, stability assays, amino acid sequencing and alignment with other protein sequences confirmed that CeEI is a member of the soybean Kunitz trypsin inhibitor family. Like other members of this family, CeEl is a 20 kDa monomeric protein; it is stable within a large pH and temperature range, with four cysteine residues forming two disulfide bridges, conserved amino acid residues and leucine-isoleucine residues in the reactive site. CeEI was able to inhibit NE and Cat G at a nanomolar range (with K(i)s of 1.9 and 3.6 nM, respectively) and inhibited PR3 within a micromolar range (K-i 3.7 mu M), leading to hydrolysis of specific synthetic substrates. in a lung edema model, CeEI reduced the lung weight and pulmonary artery pressure until 180 min after the injection of zymosan-activated polymorphonuclear neutrophils. in experiments performed in the presence of a Cat G and PR3, but not an NE inhibitor, lung edema was reduced only until 150 min and pulmonary artery pressure was similar to that of the control. These results confirm that NE action is crucial to edema establishment and progression. Additionally, CeEI appears to be a useful tool for studying the physiology of pulmonary edema and provides a template for molecular engineering and drug design for ALI therapy. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Giessen, Dept Internal Med, D-35392 Giessen, Hesse, Germany
dc.description.affiliationUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 Santos, SP, Brazil
dc.description.affiliationUniv São Paulo, Sch Arts Sci & Humanities, BR-03828000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationInst Bot, Sect Physiol & Biochem Plants, BR-01061970 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundo de Auxilio a Pesquisa da Universidade Federal de São Paulo (FAP/UNIFESP), Brazil
dc.format.extent235-243
dc.identifierhttp://dx.doi.org/10.1016/j.phytochem.2013.09.025
dc.identifier.citationPhytochemistry. Oxford: Pergamon-Elsevier B.V., v. 96, p. 235-243, 2013.
dc.identifier.doi10.1016/j.phytochem.2013.09.025
dc.identifier.issn0031-9422
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37045
dc.identifier.wosWOS:000328869500023
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPhytochemistry
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCaesalpinia echinata Lamen
dc.subjectFabaceaeen
dc.subjectPulmonary edemaen
dc.subjectProtease inhibitorsen
dc.subjectCaesalpinia echinata elastase inhibitoren
dc.subjectSeedsen
dc.titleUsing a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edemaen
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