Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients

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dc.contributor.authorNoronha, Samuel Marcos Ribeiro de [UNIFESP]
dc.contributor.authorCorrêa, Silvana Aparecida Alves [UNIFESP]
dc.contributor.authorKlepp, Anthony Gueratto
dc.contributor.authorIpolito, Michele Zampieri
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.authorGragnani, Alfredo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:52Z
dc.date.available2015-06-14T13:46:52Z
dc.date.issued2014-01-01
dc.description.abstractPURPOSE:To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn.METHODS:Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine (r) Human KGF. The experiments were performed in triplicate.RESULTS:The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL- 6, IL- 10, and IL- 1beta were observed no difference. For IL - 8, TNF - alpha and KGF was observed a significant difference between the expression in large and small burned patient.CONCLUSION:That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients.en
dc.description.affiliationUNIFESP-EPM Department of Surgery
dc.description.affiliationUNIFESP-EPM
dc.description.affiliationUNIFESP-EPM Department of Gynecology
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM) Department of Surgery
dc.description.affiliationUnifespUNIFESP, EPM, Department of Surgery
dc.description.affiliationUnifespUNIFESP-EPM
dc.description.affiliationUnifespUNIFESP, EPM Department of Gynecology
dc.description.affiliationUnifespUNIFESP, EPM, Department of Surgery
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2011/12945-4
dc.description.sponsorshipIDFAPESP: 2011/23.985-7
dc.format.extent62-68
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001300012
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 62-68, 2014.
dc.identifier.doi10.1590/S0102-86502014001300012
dc.identifier.fileS0102-86502014001300013.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001300013
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8178
dc.identifier.wosWOS:000342065000013
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsAcesso aberto
dc.subjectBurnsen
dc.subjectFibroblastsen
dc.subjectFlow Cytometryen
dc.subjectImmunoenzyme Techniqueen
dc.subjectFibroblast Growth Factor 7en
dc.subjectInterleukinsen
dc.subjectTumor Necrosis Factor Alphaen
dc.titleKeratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patientsen
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