Muscarinic acetylcholine receptor subtypes in the rat seminal vesicle

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dc.contributor.authorHamamura, M.
dc.contributor.authorMarostica, E.
dc.contributor.authorAvellar, MCW de
dc.contributor.authorPorto, C. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:02Z
dc.date.available2016-01-24T12:41:02Z
dc.date.issued2006-03-09
dc.description.abstractThe aim of the present study was to identify the muscarinic acetylcholine receptor (mAChR) mRNA subtypes in the rat seminal vesicle. Furthermore, the mAChR subtypes involved in the contraction of the seminal vesicle were also explored. Reverse transcriptase-polymerase chain reaction (PCR) was performed and five PCR products corresponding to M-1-M-5 mAChR mRNA subtypes were detected in this tissue. Functional pharmacological studies indicated that the rank order of mAChR antagonists in blocking the contractile effects of carbachol was p-fluoro-hexahydro-sila-difenidol (pF-HHSiD) >> tropicamide > methoctramine = pirenzepine. This antagonist profile indicates that M-3 mAChR subtype is predominantly involved in the seminal vesicle contraction. Furthermore, immunohistochemical studies confirmed the presence of the M-3 mAChR subtype in the smooth muscle layers. M-2 mAChR subtype was also immunolocalized in smooth muscle cells and may be involved in the contraction of this tissue. the presence of M-2 and M-3 mAChR subtypes in the epithelial cells suggests that these receptors could be involved in the protein secretion. Taken together, the cholinergic neurotransmitter may be a factor controlling contractility and protein secretion in this tissue. (c) 2006 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent192-198
dc.identifierhttp://dx.doi.org/10.1016/j.mce.2006.01.004
dc.identifier.citationMolecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 247, n. 1-2, p. 192-198, 2006.
dc.identifier.doi10.1016/j.mce.2006.01.004
dc.identifier.issn0303-7207
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28791
dc.identifier.wosWOS:000236313900022
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectseminal vesicleen
dc.subjectmuscarinic acetylcholine receptor subtypesen
dc.subjectmale reproductive tracten
dc.titleMuscarinic acetylcholine receptor subtypes in the rat seminal vesicleen
dc.typeArtigo
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