TGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding

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dc.contributor.authorAlves, Claudia Cristina
dc.contributor.authorTorrinhas, Raquel Susana
dc.contributor.authorGiorgi, Ricardo
dc.contributor.authorBrentani, Maria Mitzi
dc.contributor.authorLogullo, Angela Flavia [UNIFESP]
dc.contributor.authorWaitzberg, Dan Linetzky
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:59Z
dc.date.available2016-01-24T14:37:59Z
dc.date.issued2014-10-01
dc.description.abstractMalnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. in experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets.en
dc.description.affiliationUniv São Paulo, Med Sch FMUSP, Dept Gastroenterol, Lab Nutr & Metab Surg Digest Syst LIM 35, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, NAPAN, Food & Nutr Res Ctr, São Paulo, Brazil
dc.description.affiliationFMUSP, Dept Internal Med, Human Nutr & Metab Dis Lab LIM 25, São Paulo, Brazil
dc.description.affiliationFMUSP São Paulo, Dept Radiol, Expt Oncol Lab LIM 24, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med UNIFESP EPM, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med UNIFESP EPM, Dept Pathol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Apoio a Pesquisa do Estado de São Paulo
dc.description.sponsorshipIDFundacao de Apoio a Pesquisa do Estado de São Paulo: Fapesp 05/54185-5
dc.format.extent533-539
dc.identifierhttp://dx.doi.org/10.1111/j.1742-481X.2012.01120.x
dc.identifier.citationInternational Wound Journal. Hoboken: Wiley-Blackwell, v. 11, n. 5, p. 533-539, 2014.
dc.identifier.doi10.1111/j.1742-481X.2012.01120.x
dc.identifier.issn1742-4801
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38310
dc.identifier.wosWOS:000342664900012
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Wound Journal
dc.rightsAcesso restrito
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectAntioxidantsen
dc.subjectArginineen
dc.subjectEnteral nutritionen
dc.subjectGrowth factorsen
dc.subjectTGF-(1)en
dc.subjectWound healingen
dc.titleTGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feedingen
dc.typeArtigo
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