Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

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dc.contributor.authorSilva Pinhal, Maria Aparecida [UNIFESP]
dc.contributor.authorLeal Almeida, Maria Carolina [UNIFESP]
dc.contributor.authorCosta, Alessandra Scorse [UNIFESP]
dc.contributor.authorTheodoro, Therese Rachell [UNIFESP]
dc.contributor.authorSerrano, Rodrigo Lorenzetti [UNIFESP]
dc.contributor.authorSantos Machado Filho, Carlos D'Apparecida [UNIFESP]
dc.date.accessioned2019-07-22T15:46:44Z
dc.date.available2019-07-22T15:46:44Z
dc.date.issued2016
dc.description.abstractBackground: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Objectives: Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). Methods: Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). Results: The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. Conclusion: The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.en
dc.description.affiliationFac Med ABC, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Sao Paulo, SP, Brazil
dc.description.affiliationFac Med ABC, Santo Andre, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
dc.description.sponsorshipCNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologia)
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
dc.format.extent595-600
dc.identifierhttp://dx.doi.org/10.1590/abd1806-4841.20164957
dc.identifier.citationAnais Brasileiros De Dermatologia. Rio De Janeiro Rj, v. 91, n. 5, p. 595-600, 2016.
dc.identifier.doi10.1590/abd1806-4841.20164957
dc.identifier.fileS0365-05962016000500595.pdf
dc.identifier.issn0365-0596
dc.identifier.scieloS0365-05962016000500595
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51050
dc.identifier.wosWOS:000388465100005
dc.language.isoeng
dc.publisherSoc Brasileira Dermatologia
dc.rightsAcesso aberto
dc.subjectCarcinomaen
dc.subjectbasal cellen
dc.subjectCarcinomaen
dc.subjectsquamous cellen
dc.subjectGlycosaminoglycansen
dc.subjectHyaluronic aciden
dc.subjectNeoplasmsen
dc.subjectPolymerase chain reactionen
dc.subjectSkin neoplasmsen
dc.titleExpression of heparanase in basal cell carcinoma and squamous cell carcinomaen
dc.typeArtigo
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