Avaliação de ligas de cobre como agentes desestabilizantes de biofilme bacteriano: uma perspectiva de sua aplicação contra a poluição ambiental
Data
2023-01-04
Autores
Almeida, Juliana Bertolino Sena de [UNIFESP]
Orientadores
Vasconcellos, Suzan Pantaroto de [UNIFESP]
Tipo
Trabalho de conclusão de curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Resumo
O cobre (Cu) é um elemento conhecido por sua importância, como, micronutriente
para o bom funcionamento de metabolismo de seres vivos, outra característica desse
elemento é a presença de atividade microbicida, característica essa que já é
conhecida e empregada a milhares de anos. Essa utilização se dá por esse elemento
apresentar taxas de toxicidade a microrganismos quando em exposição prolongada e
altas concentrações, causando interferências estruturais e metabólicas nesses
microrganismos. Atualmente o elemento já vem sendo utilizado como parte da
composição de alguns materiais como tintas, fibras de roupas, com o intuito de trazer
uma maior proteção contra microrganismos patogênicos. Ao longo dos últimos anos,
é observado um aumento do fator de virulência de bactérias, tornando os antibióticos
e soluções sanitizantes menos eficientes no combate a esses microrganismos, e com
isso a necessidade de buscar novos meios de maior eficiência para essa eliminação.
Nesse estudo, a partir das análises de absorbância dos cupons de Cu inoculados com
a bactéria Pseudomonas aeruginosa foi identificado que cupons de Cu inoculados
com a bactéria apresentaram atividade inibidora da formação de biofilme após 14 dias
em contato com os cupons, assim como, a solução de Acetato de Cobre II Cu
(CH3COO)2, apresentou um potencial parcial de atividade inibidora de crescimento de
biofilme após 14 dias em contato com os cupons. Após a realização das análises e
identificação do seu real potencial de inibição do crescimento de biofilme da bactéria
Pseudomonas aeruginosa em sua superfície, foram trazidas perspectivas da utilização
desse material e solução como agente biocida para a não formação e eliminação de
biofilme em tubulações industriais. Como forma de evitar processos de bioincrustação,
os quais acarretam em vazamentos e rompimento de dutos, trazendo prejuízos
econômicos e ambientais como a contaminação de solo, água, populações humanas,
assim como da fauna e flora.
Copper (Cu) is an element known for its importance, as a micronutrient for the proper functioning of the metabolism of living beings, another characteristic of this element is the presence of microbicidal activity, a characteristic that has been known and used for thousands of years. This use is due to this element presenting toxicity rates to microorganisms when exposed to prolonged exposure and high concentrations, causing metabolic and metabolic interference in these microorganisms. Currently, the element is already being used as part of the composition of some materials such as paints, clothing fibers, in order to provide greater protection against pathogenic microorganisms. Over the last few years, an increase in the virulence factor of bacteria has been observed, making antibiotics and sanitizing solutions less efficient in combating these microorganisms, and with that the need to seek new, more efficient means for their elimination. In this study, based on absorbance analyzes of Cu coupons inoculated with the bacterium Pseudomonas aeruginosa, it was identified that Cu coupons inoculated with the bacteria showed inhibiting activity in biofilm formation after 14 days in contact with the coupons, as well as the solution of Copper II Cu Acetate (CH3COO)2, showed a potential for partial inhibition of the biofilm growth activity after 14 days in contact with the coupons. After completing the analyzes and identifying its real potential for advancing the growth of Pseudomonas aeruginosa biofilm on its surface, perspectives were brought for the use of this material and solution as a biocide agent to prevent the formation and elimination of biofilm in industrial pipes. As a way to avoid biofouling processes, which lead to leaks and rupture of pipelines, causing biological and environmental damage, such as contamination of soil, water, humans, as well as fauna and flora.
Copper (Cu) is an element known for its importance, as a micronutrient for the proper functioning of the metabolism of living beings, another characteristic of this element is the presence of microbicidal activity, a characteristic that has been known and used for thousands of years. This use is due to this element presenting toxicity rates to microorganisms when exposed to prolonged exposure and high concentrations, causing metabolic and metabolic interference in these microorganisms. Currently, the element is already being used as part of the composition of some materials such as paints, clothing fibers, in order to provide greater protection against pathogenic microorganisms. Over the last few years, an increase in the virulence factor of bacteria has been observed, making antibiotics and sanitizing solutions less efficient in combating these microorganisms, and with that the need to seek new, more efficient means for their elimination. In this study, based on absorbance analyzes of Cu coupons inoculated with the bacterium Pseudomonas aeruginosa, it was identified that Cu coupons inoculated with the bacteria showed inhibiting activity in biofilm formation after 14 days in contact with the coupons, as well as the solution of Copper II Cu Acetate (CH3COO)2, showed a potential for partial inhibition of the biofilm growth activity after 14 days in contact with the coupons. After completing the analyzes and identifying its real potential for advancing the growth of Pseudomonas aeruginosa biofilm on its surface, perspectives were brought for the use of this material and solution as a biocide agent to prevent the formation and elimination of biofilm in industrial pipes. As a way to avoid biofouling processes, which lead to leaks and rupture of pipelines, causing biological and environmental damage, such as contamination of soil, water, humans, as well as fauna and flora.