Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines
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2016
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Calcagno, Danielle Queiroz [UNIFESP]
Takeno, Sylvia Santomi [UNIFESP]
Gigek, Carolina Oliveira [UNIFESP]
Leal, Mariana Ferreira [UNIFESP]
Wisnieski, Fernanda [UNIFESP]
Chen, Elizabeth Suchi [UNIFESP]
Araújo, Taíssa Maíra Thomaz [UNIFESP]
Lima, Eleonidas Moura [UNIFESP]
Melaragno, Maria Isabel [UNIFESP]
Demachki, Samia [UNIFESP]
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AIM To identify common copy number alterations on gastric cancer cell lines. METHODS Four gastric cancer cell lines (ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis. RESULTS The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha (IL11RA) and maternal embryonic leucine zipper kinase (MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11RA and MELK was observed in 19.1% (13/68) and 55.9% (38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.
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World Journal Of Gastroenterology. Pleasanton, v. 22, n. 43, p. 9506-9514, 2016.