Kinetic Modeling of Ethanol Production by Scheffersomyces stipitis from Xylose
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2014-01-01
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Farias, Daniele
Andrade, Rafael R. de [UNIFESP]
Maugeri-Filho, Francisco
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This work focuses on the kinetics of ethanol production by Scheffersomyces stipitis on xylose with the development of a mathematical model considering the effect of substrate and product concentrations on growth rate. Experiments were carried out in batch and continuous modes, with substrate concentration varying from 7.2 to 145 g L-1. Inhibitory effects on cell growth, substrate uptake, and ethanol production rates were found to be considerable. Kinetic parameters were obtained through linear and non-linear regression methods. Experiments in continuous mode were performed at different dilution rates to evaluate the inhibitory effect of ethanol. A mixed mathematical model which combined Andrews and Levenspiel's models, combining substrate and product inhibition, was used. A quasi-Newton routine was applied to obtain a more accurate fitting of kinetic parameters. the parameters such as cell to product factor (Y (P/X)) and limiting cell yield (Y (X)) were shown to be dependent on substrate concentration. the kinetic model fitted satisfactorily the experimental data.
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Applied Biochemistry and Biotechnology. Totowa: Humana Press Inc, v. 172, n. 1, p. 361-379, 2014.