Estudo morfológico do intestino delgado de ratos na isquemia-reperfusão sob oxigenação hiperbárica
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Data
2005
Autores
Bertoletto, Paulo Roberto [UNIFESP]
Orientadores
Fagundes, Djalma José [UNIFESP]
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
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Objetivo: Estudar aspectos morfológicos e imuno-histoquímicos do intestino delgado de ratos
sob isquemia e reperfusão (I/R) e submetidos à oxigenação hiperbárica. Métodos: Trinta e
dois ratos foram distribuídos em quatro grupos (n=8) nos quais foi realizada a isquemia de
sessenta minutos e avaliados após reperfusão de sessenta minutos. Nos animais do grupo I não
foi realizado nenhum procedimento de oxigenação hiperbárica (OHB), no grupo II a OHB foi
realizada no período de isquemia, no grupo II a OHB foi realizada no período de reperfusão e
no grupo IV foi realizada nos períodos de isquemia e reperfusão. Foi usada uma câmara
especial de acrílico para animais de pequeno porte com 2,0 atmosferas de pressão e oxigênio a
100% para os procedimentos de OHB. Segmentos de cinco centímetros de íleo terminal foram
coletados após sessenta minutos de reperfusão para estudo histológico e imuno-histoquímico,
sendo avaliados os graus de lesão da mucosa intestinal (ausente, leve, moderada e acentuada),
espessura da camada mucosa e do índice de apoptose pela caspase 3. Resultados: O grupo I
apresentou lesões acentuadas da mucosa, diminuição da espessura da camada mucosa e alto
índice de apoptose (GI=0,70), caracterizando um modelo de lesão de I/R. O grupo II
apresentou graus de lesões de mucosa ausentes ou leves e menor índice de
apoptose(GII=0,16). Os resultados dos grupos I e III são semelhantes com grau de lesões
moderadas ou acentuadas e diminuição da espessura da camada mucosa e índice de apoptose
próximo (GI= 0,70 e GII=0,84). No grupo IV os graus de lesões foram semelhantes ao grupo
II, porém, com maior índice de apoptose (GII=0,16 e GIV=0,42). Conclusão: A oxigenação
hiperbárica realizada no período de isquemia preveniu as lesões de I/R de modo mais eficaz
do que aplicada no período de isquemia e reperfusão. Quando aplicada somente no período de
reperfusão os resultados foram piores sugerindo que seu efeito protetor esteja vinculado a
prevenção da formação dos radicais livres no período de isquemia.
Objective: To study morphologic and immune-hystochemical aspects of the small intestine of rats under ischemia and reperfusion (I/R) and submitted to the hyperbaric oxygenation (HBO). Methods: Thirty two rats were distributed in four groups (n=8) us which the ischemia of sixty minutes was accomplished and appraised after reperfusion of sixty minutes. In the animals of the group I any procedure of HBO was not accomplished, in the group II HBO was accomplished in the ischemia period, in the group II HBO was accomplished in the reperfusion period and in the group IV it was accomplished in the ischemia periods and reperfusion. A special acrylic chamber was used with 2.0 pressure atmospheres and oxygen to 100% for the procedures of HBO. Segments of five centimeters of terminal ileum were collected after sixty minutes of reperfusion for histological study and immune-hystochemical, being appraised the degrees of lesion of the intestinal mucosa (absent, mild, moderate and severe), thickness of the mucosa and of the apoptosis index for the caspase 3. Results: The group I presented accentuated lesions of the mucosa, decrease of the thickness of the mucosa layer and high apoptosis index (GI=0.70), characterizing a model of lesion of I/R. The group II presented degrees lesions of mucosa absent or mild and smaller apoptosis (GII=0.16). The results of the groups I and III are similar with degree of moderate or accentuated lesions and decrease of the thickness of the mucosa and index of similar apoptosis (GI = 0.70 and GII=0.84). In the group IV the degrees of lesions were similar to the group II, however, with larger apoptosis index (GII=0.16 and GIV=0.42). Conclusions: The hyperbaric oxygenation accomplished in the ischemia period prevented the lesions of I/R in way more effective than applied in the ischemia period and reperfusion. When only applied in the reperfusion period the results were worse suggesting that the protecting effect is linked the prevention of the formation of the free radicals in the ischemia period.
Objective: To study morphologic and immune-hystochemical aspects of the small intestine of rats under ischemia and reperfusion (I/R) and submitted to the hyperbaric oxygenation (HBO). Methods: Thirty two rats were distributed in four groups (n=8) us which the ischemia of sixty minutes was accomplished and appraised after reperfusion of sixty minutes. In the animals of the group I any procedure of HBO was not accomplished, in the group II HBO was accomplished in the ischemia period, in the group II HBO was accomplished in the reperfusion period and in the group IV it was accomplished in the ischemia periods and reperfusion. A special acrylic chamber was used with 2.0 pressure atmospheres and oxygen to 100% for the procedures of HBO. Segments of five centimeters of terminal ileum were collected after sixty minutes of reperfusion for histological study and immune-hystochemical, being appraised the degrees of lesion of the intestinal mucosa (absent, mild, moderate and severe), thickness of the mucosa and of the apoptosis index for the caspase 3. Results: The group I presented accentuated lesions of the mucosa, decrease of the thickness of the mucosa layer and high apoptosis index (GI=0.70), characterizing a model of lesion of I/R. The group II presented degrees lesions of mucosa absent or mild and smaller apoptosis (GII=0.16). The results of the groups I and III are similar with degree of moderate or accentuated lesions and decrease of the thickness of the mucosa and index of similar apoptosis (GI = 0.70 and GII=0.84). In the group IV the degrees of lesions were similar to the group II, however, with larger apoptosis index (GII=0.16 and GIV=0.42). Conclusions: The hyperbaric oxygenation accomplished in the ischemia period prevented the lesions of I/R in way more effective than applied in the ischemia period and reperfusion. When only applied in the reperfusion period the results were worse suggesting that the protecting effect is linked the prevention of the formation of the free radicals in the ischemia period.
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Citação
São Paulo: [s.n.], 2005. 70 p.