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dc.contributor.authorBanin, Renata Mancini [UNIFESP]
dc.contributor.authorHirata, Bruna Kelly Sousa [UNIFESP]
dc.contributor.authorAndrade, Iracema Senna de [UNIFESP]
dc.contributor.authorZemdegs, Juliane Costa Silva [UNIFESP]
dc.contributor.authorClemente, Ana Paula Grotti [UNIFESP]
dc.contributor.authorDornellas, Ana Paula Segantine [UNIFESP]
dc.contributor.authorBoldarine, Valter Tadeu [UNIFESP]
dc.contributor.authorEstadella, Debora [UNIFESP]
dc.contributor.authorAlbuquerque, Kelse Tibau de [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.authorTelles, Monica Marques [UNIFESP]
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 9, p. 780-788, 2014.
dc.description.abstractGinkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats gavaged with 500 mg/kg of GbE diluted in the saline vehicle (HFD+Gb). NFD rats were gavaged with the saline vehicle only. At the end of the treatment, the rats were anesthetized, insulin was injected into the portal vein, and after 90s, the gastrocnemius muscle was removed. The quantification of IRS-1, Akt, and Akt phosphorylation was performed using Western blotting. Serum levels of fasting insulin and glucose, triacylglycerols and total cholesterol, and LDL and HDL fractions were measured. An insulin tolerance test was also performed. Ingestion of a hyperlipidic diet promoted loss of insulin sensitivity and also resulted in a significant increase in body adiposity, plasma triacylglycerol, and glucose levels. In addition, GbE treatment significantly reduced food intake and body adiposity while it protected against hyperglycemia and dyslipidemia in diet-induced obesity rats. It also enhanced insulin sensitivity in comparison to HFD+V rats, while it restored insulin-induced Akt phosphorylation, increased IRS-1, and reduced PTP-1B levels in gastrocnemius muscle. The present findings suggest that G. biloba might be efficient in preventing and treating obesity-induced insulin signaling impairment.en
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectGinkgo biloba extracten
dc.subjectInsulin toleranceen
dc.subjectBody adiposityen
dc.subjectAkt phosphorylationen
dc.titleBeneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese ratsen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Alagoas Faculdade de Nutrição
dc.contributor.institutionUniversidade Federal do Rio de Janeiro Curso de Nutrição
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Ciências Biológicas
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Fisiologia Disciplina de Fisiologia da Nutrição
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Biociências
dc.description.affiliationUniversidade Federal de Alagoas Faculdade de Nutrição
dc.description.affiliationUniversidade Federal do Rio de Janeiro Curso de Nutrição
dc.description.affiliationUnifespUNIFESP, Depto. de Ciências Biológicas
dc.description.affiliationUnifespUNIFESP, Depto. de Fisiologia Disciplina de Fisiologia da Nutrição
dc.description.affiliationUnifespUNIFESP, Depto. de Biociências
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