Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/xmlui/handle/11600/8038
Title: Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats
Authors: Estevão, Lígia Reis Moura
Medeiros, Juliana Pinto de
Baratella-Evêncio, Liriane
Simões, Ricardo Santos [UNIFESP]
Mendonça, Fábio de Souza
Evêncio-Neto, Joaquim
Federal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal Bioscience
UFRPE Department of Morphology and Physiology Histology Division
Federal University of Pernambuco Department of Morphology Histology Division
Universidade Federal de São Paulo (UNIFESP)
Keywords: Surgical Flaps
Fabaceae
Skin
Ischemia
Rats
Issue Date: 1-Dec-2013
Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Citation: Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 12, p. 863-869, 2013.
Abstract: PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.
URI: http://repositorio.unifesp.br/handle/11600/8038
ISSN: 0102-8650
Other Identifiers: http://dx.doi.org/10.1590/S0102-86502013001200009
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