Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/55919
Title: Sleep deprivation predisposes allergic mice to neutrophilic lung inflammation
Authors: Nunes, Jethe O. F. [UNIFESP]
Apostolico, Juliana de Souza [UNIFESP]
Andrade, David Anibal Garrido [UNIFESP]
Ruiz, Francieli Silva [UNIFESP]
Fernandes, Edgar R. [UNIFESP]
Andersen, Monica Levy [UNIFESP]
Keller, Alexandre Castro [UNIFESP]
Rosa, Daniela Santoro [UNIFESP]
Keywords: Sleep deprivation
allergic lung inflammation
airway neutrophilia
IL-17
mouse model
Issue Date: 2018
Publisher: Mosby-Elsevier
Citation: Journal Of Allergy And Clinical Immunology. New York, v. 141, n. 3, p. 1018-+, 2018.
Abstract: Background: Although different studies associated sleep deprivation (SD) with systemic inflammatory changes, the effect of sleep duration on the pathology of allergic chronic diseases is poorly understood. Objective: We sought to evaluate the influence of SD on allergen-induced pulmonary inflammation. Methods: Ovalbumin (OVA)-sensitized C57BL/6 mice were exposed to a first set of intranasal OVA challenge under SD or healthy sleep (HS) conditions, followed by a second OVA challenge, 1 week apart. Some groups were subjected to corticosteroid treatment with dexamethasone. Results: OVA-sensitized mice with SD had more severe airway inflammation than the allergic group with HS. Analysis of lung parenchyma revealed that the inflammation in allergic mice with SD was marked by an influx of neutrophils (mainly) and eosinophils and secretion of IL-6, TNF-alpha, and IL-17 in contrast to the eosinophilic inflammation and IL-4 production observed in allergic mice with HS. The same cytokine profile was observed in ex vivo culture of cervical lymph node cells and splenocytes, indicating that in allergic mice SD favors immune responses toward a proinflammatory TH17 profile. This idea is supported by the fact that disruption of IL-17 signaling (IL-17 receptor A(-/-)) prevented airway neutrophilia in allergic mice with SD. Furthermore, allergic mice with SD became refractory to corticosteroid treatment in contrast to the allergic group with HS. Conclusion: Collectively, our data show that sleep quality participates in the progression of allergen-induced eosinophilic lung inflammation to corticosteroid-refractory neutrophilic manifestation.
URI: https://repositorio.unifesp.br/handle/11600/55919
ISSN: 0091-6749
Other Identifiers: http://dx.doi.org/10.1016/j.jaci.2017.06.025
Appears in Collections:Artigo
Artigo
Artigo

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.